Effects of Processing Conditions on the Protein Quality of Fried Anchovy Kamaboko Engraulis japonica

  • cc icon

    The effects of processing and frozen storage conditions on the quality of anchovy Engraulis japonica fried surimi gels were investigated. Protein content decreased after surimi gel processing from 19.6% (raw meat) to 12.1% (kamaboko) due to the added ingredients and change in water content. Lipid content decreased from 2.8% (raw meat) to 1.3% in minced and 0.5% in surimi, but fried kamaboko showed a 6.9 % lipid level. Thiobarbituric acid values and thiobarbituric acid reactive substances levels were highest in kamaboko samples, 89.5 and 1.9 mg/g solid, and increased gradually with storage time to 101.8 and 4.6 mg/g solid, respectively. In vitro protein digestibility increased from 79.2% in raw anchovy to 88.5% in kamaboko samples. Levels of trypsin inhibitor decreased gradually with processing and during storage time from 2.43 in raw anchovy to 0.31 mg/g solid in the kamaboko sample after 60 days of frozen storage. No noticeable changes in total essential amino acid was observed during processing conditions. Computed protein efficiency ratio for kamaboko was highest (2.59) compared with whole anchovy (1.96), minced (1.94) and surimi (2.50). Fresh fried anchovy kamaboko showed similar values of hardness, springiness, gumminess and chewiness to commercial surimi gel, but a higher values were seen for fracturability and adhesiveness, and lower values for cohesiveness and resilience. The frozen and thawed anchovy kamaboko showed higher values for all of these rheological parameters compared with fresh and commercial kamaboko. Anchovy kamaboko showed the lowest lightness (62.9) and redness (0.16) and similar yellowness (11.9) compared with commercial kamaboko. Frozen storage and vacuum packaging were effective maintaining the shelf life of anchovy kamaboko within 30 days, but were not effective after 45 days due to fat oxidation.


    Kamaboko , Engraulis japonica , Processing methods , Protein quality , Color , Texture profile

  • Introduction

    The anchovy Engraulis ringens is a small pelagic fish and the most important resource in Peru’s fishing grounds. Approximately 97% of the annual landing of anchovy stocks is used in the manufacture of indirect human consumption products and only 3% is used for direct human consumption. Recently on a small scale, it has been processed, cured, and frozen, highlighting potentially greater use by the canning industry in different presentations due to the scarcity of resources such as sardine; this has increased noticeably since 2006 (Perez et al., 2010). According to these data, a possibility exists of developing new products based on this species. Indeed, surimi is an excellent high-protein base for a new generation of foods (Pigott and Tucker, 1990).

    Since the first preparation of frozen surimi, surimi has been used in the preparation of kamaboko and many kinds of value added seafood products. Making kamabo, mainly from muscle protein, raises many interesting issues from the perspective of protein chemistry (Suzuki, 1981).

    Variation in the levels of nutrients in raw material is due to more than just processing. Some benefits of processing are the destruction of anti-nutritional factors, such as amylase and trypsin inhibitors and increased digestibility of starch and protein (Pigott and Tucker, 1990). Protein represents one of the most valuable components of our diet and should be protected during food processing. The maintenance of protein nutritive value is particularly important during the production of meat products because meat proteins are balanced, containing all essential amino acids. The nutritive value of meat proteins is determined not only by the quantitative and qualitative composition of the amino acids, but also by their availability to digestive tract proteolytic enzymes (Korczak et al., 2004). Important chemical reactions that decrease the nutritional value of seafood are lipid oxidation and nonenzymatic browning (Pigott and Tucker, 1990). Reduced digestibility and limitations as to the amount and the degree of amino acid availability are affected by the formation of amino acid bonds with lipid oxidation products. Fat oxidation products react first with functional groups of proteins and amino acids; oxidized lipid effects are a major cause of rancidity formation in food products, but oxidized lipids also interact with other food components and change nutritional value (Korczak et al., 2004).

    An important and frequently observed effect in food processing is a reduction in protein nutritive quality. These changes may depend on denaturation of the proteins and reduced amino acid availability as a result of cross-linking, racemization, degradation and the formation of complexes with sugars and may result in loss of digestibility. Thus, when attempting to estimate protein quality, one of the first factors that must be evaluated is digestibility. Because the nutritional quality of a protein is related both to its amino acid content and the capacity of digestive enzymes to liberate them, methods using digestive enzymes have been attempted (Gauthier et al., 1982).

    In this investigation, we sought to assess the possibility of using anchovy catches for kamaboko products and to study the food qualities of those products including an evaluation of protein quality. To compare the food qualities of anchovy kamaboko with commercial kamaboko from white muscle fishes, protein nutritional value, degree of lipid oxidation, textural properties, and color value were determined using fried kamaboko products from anchovy Engraulis japonica surimi.

    Materials and Methods

      >  Sample preparation

    In total, 80 kg of fresh anchovy samples were obtained as quick frozen blocks in Gijang Market, Busan South Korea, on January 19, 2012. Average sizes of whole anchovies were 14.3 ± 0.48 cm, in total length, 12.5 ± 0.44 cm in standard length, and 18.1 ± 2.28 g in weight.

    Each frozen anchovy block was divided into individual quick frozen samples, and those samples were lightly thawed at room temperature for 5 min and then headed and gutted. Skin and backbone samples were removed to obtain the muscle. Minced meat was obtained from the muscle using a meat chopper (M-125 FUJEE; Hankook Fujee Industries Co. Ltd., Hwaseong, Korea). Then the samples were weighed and washed with cool water (5℃) in a 3:1 water/minced fish ratio. The minced fish was washed three times, the first wash with 0.5 NaHCO3 solution, and the last wash water had 0.3% NaCl, added; after each washing, excess water was removed by pressing.

    Surimi was prepared by mixing 4% sucrose, 4% sorbitol and 0.3% polyphosphate and storage under freezing conditions (Maza Ramirez and Llave Perez, 2006).

    Kamaboko base was prepared by mixing surimi (48.4%), salt (2.9%), sugar (1.7%), soybean oil (2.5%), monosodium glutamate (MSG; 1.0%), white egg (3.5%), mirin (2.0%), wheat flour (8.0%), and cool water (30.0%). Mixed kamaboko base was formed into thin slices of 5.50 ± 0.66 mm in thickness and fried in 180℃ boiling soybean oil for 3 min. Fried kamaboko was stored under freezing conditions (-24℃) in a vacuum, pack for 0, 15, 30, 45, and 60 days. Whole anchovy (raw material), ordinary muscle (minced), and surimi samples were also used to study the effects of frozen storage on food quality.

      >  Experimental procedures

    Proximate composition

    The proximate compositions of the all samples were determined using the following Association of Official Analytical Chemist (AOAC, 1990) procedure. Moisture (%) samples were dried in a drying oven at 105℃ to constant weight. Crude fat (%) was determined using a Soxhlet solvent extractor. Crude protein (%) was determined by the semi-micro Kjeldahl method using 6.25 as a conversion factor after acid digestion. Crude ash (%) was determined by incineration in an electric muffle furnace (250℃ for 2 h and 570℃ for 1 h).

    The percentage contents of protein, fat, and ash were determined in relation to the dry basis of the samples analyzed, dry basis being expressed in g × 100. The carbohydrate contents were calculated by the difference in proximate composition. All determinations were made in triplicate for each run.

    Water activity measurements

    Water activity measurements were taken for all the samples, using the water activity-measuring equipment (BT-RSI-7557 012; Rotronic, Grindelwald, Switzerland).

    Fat oxidation

    To measure fat oxidation, two tests were used with all samples to estimate the extent of oxidation.

    Thiobarbituric acid value (TBA value). TBA values were determined by the method of Tarladgis et al. (1960). Absorbance was measured in an ultraviolet (UV) spectrophotometer at 538 nm and the TBA values were calculated by multiplying the sample absorbance by 100 and are expressed in mg/g solid.

    Thiobarbituric acid reactive substances (TBARs). TBARS were determined by the method of Witte et al. (1970). Absorbance was measured using a UV spectrophotometer at 530 nm and the resulting colors in all the samples were calculated by multiplying the absorbance by 5.2 and are expressed in mg/g solid.

    Protein quality determination

    In vitro protein digestibility. The in vitro protein digestibility values of all samples were determined by the method of Oduro et al. (2011) with modification by the AOAC method (1982); the procedure used the four enzymes method.

    α-Chymotrypsin (38 units/mg solid; Sigma, St. Louis, MO, USA), trypsin (13,390 BAEE units/mg solid; Sigma), and protease (Streptomyces griceus, 46 units/mg solid; Sigma) were used in the three enzymes method. The reference protein used was Animal Nutrition Research Council casein. Digestibility was calculated as follows:

    Digestibility (%, three enzymes) = 234.84 - 22.56x,

    where x is the pH of sample at 20 min.

    Digestibility (%, four enzymes) = 1.03x (three enzymes digestibility) - 0.34.

    Amino acid profile. Amino acid profiles were determined using the 6 N HCl hydrolysis method with an amino acid analyzer (S433; Sykum, Eresing, Germany). Tryptophan was determined using the 5 N NaOH alkaline hydrolysis method of Hugli and Moore (1972).

    Trypsin inhibitor (TI) assay. TI concentrations in all samples were determined using the procedure of Ryu and Lee (1985), a modification of the Rhinehart (1975) method. Results of TI are expressed in TI equivalents which equal milligrams of purified soybean TI per gram of sample.

    The correlation coefficient between pH and TI content was 1.000 and the equation for the calculation is:

    y = 5.1335x - 35.339,

    where y = purified soybean TI (mg) and x is the pH at 10 min incubation.

    Computed in vitro protein quality. Computed protein efficiency ratio (C-PER) was calculated with software, using the data obtained from the in vitro protein digestibility (four enzymes) and amino acid profiles. The calculation was based on the procedure of AOAC (1982) to obtain the protein quality data compared with casein as a standard protein.

    Starch digestibility

    In vitro starch digestibility (IVSD) was determined using freeze dried kamaboko samples (50 mg/mL of 0.2 M phosphate buffer, pH 6.9) after amylolysis with 0.5 mL of pancreatic amylase (500,000 U/mg) suspension (0.44 mg/mL of 0.2 M phosphate buffer, pH 6.9) at 37℃ for 2 h. At the end of the incubation period, 4 mL of 3,5-dinitrosalicylic acid reagent was added and the mixture was boiled for 5 min. After cooling, the absorbance of the filtered solution was measured using a UV spectrophotometer at 550 nm with maltose used as the standard. IVSD was expressed as a percentage.

    Texture analysis

    Texture profile analysis was performed using a texture analyzer (TA-XT2i, Stable Microsystem, Godalming, England). Prior to testing, kamaboko samples were equilibrated to room temperature for 30 min and cut into 1.5 × 1.5 cm sections. The samples were compressed twice at 50% of initial height using a 2.0 cm diameter cylindrical probe, at a test speed 1.5 mm/s. The mechanical properties of hardness, fracturability, adhesiveness, springiness, cohesiveness, gumminess, resilience and chewiness were evaluated.

    Color measurement

    Spectral reflectance was determined using a portable colorimeter (Model JC801; Color Techno System Co. Ltd., Tokyo, Japan). The kamaboko samples without a fried surface were placed in a plastic Petri dish. The color measurement was repeated three times. L denotes lightness on a 0 to 100 scale from black to white; a* denotes (+) red or (-) green; and b* denotes (+) yellow or (-) blue.

    Statistical analyses

    Results were presented as averages and standard deviations for each sample in triplicate. Statistical analyses of data were performed using SAS software version 9.1.3 (SAS Inc., Cary, NC, USA). Tukey’s multiple range test was carried out with one-way analysis of variance (ANOVA) to determine the significance of mean (SD) differences among treatments. The significance level was set at P = 0.05 for all analyses.

    Results and Discussion

      >  Proximate composition

    Among the samples analyzed, the proximate composition (Table 1) of raw anchovy, moisture and carbohydrates were relatively similar (73.8 ± 0.39% and 0.1 ± 0.37%, respectively) to those observed by the National Fisheries Research and Development Institute (NFRDI, 2009), 73.4% and 0.3%, respectively, and the food composition table of Rural Nutrition Institute (74.8% and 0.2%, respectively) (RDA, 1991).

    The protein and ash contents of the muscle tissue of anchovy (19.6 ± 0.00% and 3.6 ± 0.06%) were slightly higher than the references cited above (NFRDI, 17.7% and RDA, 3.2%, respectively), but the lipid content (2.8 ± 0.00%) (NFRDI, 5%; RDA, 4.1%), was low probably due to the small size of the anchovies caught in the winter season. Huss (1995) reported that the chemical composition of different fish species shows variation, depending on season, migratory behavior, sexual maturation and feeding cycles. The variation in the percentage of fat is also reflected in the percentage of water because fat and water together normally constitute about 80 % of the fillet.

    The percentage of protein and moisture increased slightly in minced (20.5 ± 0.10% and 75.6 ± 0.17%, respectively), while lipid (1.3 ± 0.06%) and ash (1.8 ± 0.10%) decreased significantly after removing the skin and bones. Less fat and an altered mineral composition were reported by Pigott and Tucker (1990), cited by Huss (1995) reported that normally the skin bears about 40% of the total fat in fish. The fat cells making up the lipid depots in fatty species are typically located in subcutaneous tissue.

    Surimi showed lower protein (14.8 ± 0.16%), lipid (0.5 ± 0.04%), and ash (0.6 ± 0.06%) contents compared with minced due to the alkaline solution used for washing the minced fish. This washing changed the mineral composition, but the wash water quality and equipment type also affected the range and extent of these changes (Pigott and Tucker, 1990). Similar results (protein, 14.1; lipid, 0.5; ash, 0.7%) were observed by Maza Ramirez and Solari (2006) in anchovy refined surimi. Reduction in total ash increased protein quality and a more favorable mineral composition, and so generally, the nutritional quality was improved. Moisture content was highest (83.2 ± 0.53%), probably due to the traditional method of dewatering. Minced fish was wrapped in a tofu bag and squeezed manually. A similar value (83.13%) was observed in Alaskan pollock surimi (control sample) by Ryu et al. (1994a) during a study

    on the effects of cryoprotectants on protein quality in pollock surimi. The moisture content from the final screened product is reduced to 80-85% by pressing in a conventional screw press (Pigott and Tucker, 1990). Mixed ingredients and frying in hot oil resulted to lower moisture contents, from 64.8% to 70.8% in the kamaboko samples and they showed slightly decreased protein content (12.1% to 14.1 ± 0.07%). Commercial kamaboko showed moisture contents ranging from 54.27 ± 1.51% to 66.4 ± 0.35%; the protein content showed similar values to commercial kamaboko, which ranges from 11.2 ± 0.0% to 13.48 ± 0.0%. Pigott and Tucker (1990) reported that the lower protein content was not due so much to washing as to dilution by added carbohydrates. The lipids showed a higher level (5.5% to 6.9 ± 0.08%) than commercial kamaboko (2.8 ± 0.15% to 5.2 ± 0.26%). Oil absorption in fried foods may range from 10% to 40%, depending on the conditions of frying and the nature and size of the food (DeMan, 1999). The percentage of ash was higher (2.9% to 3.4 ± 0.16%) through the addition of some ingredients like salt compared with commercial kamaboko (2.0 ± 0.0% to 2.93 ± 0.23%). Carbohydrates (8.6% to 11.7 ± 0.92%), were increased in the kamaboko samples due to the added wheat flour, compared with commercial kamaboko ranging from 13.88 ± 0.0% to 28.26 ± 0.0%.

      >  Water activity

    The results presented in the Table 2 showed significant differences between the raw samples and fried samples. Water activity values were higher in the raw samples (0.96) and highest in the surimi (0.97), rather than the kamaboko samples (0.91-0.92) during frozen storage time.

    The aw in commercial kamaboko samples ranged from 0.92 to 0.96. Usually, the range aw 0.90-1.0 is classified as water-rich food (Pigott and Tucker, 1990) and is considered to indicate susceptibility to bacterial growth.

      >  Fat oxidation

    TBA values and TBARS are indicators of lipid oxidation or rancidity; the results in Table 3 show the degree of fat oxidation for these two assays. TBA values were relatively high in raw anchovy (136.6 ± 0.06 mg/g solid) because the anchovy is a fatty species. A lower value was seen in minced fish (41.1 ± 1.51 mg/g solid) without skin, head, viscera, or bones, and

    surimi showed the lowest value (40.0 ± 0.45 mg/g solid), probably because washing effectively removed oil from the minced fish. Washing removed about 37% of the solids, which included blood, pigments, fats, and soluble protein (Pigott and Tucker, 1990). Chen et al. (1997) observed in horse mackerel that the oil content of unwashed mince was 2.24% and decreased with washing time. The oil elimination efficiency reached 95%.

    TBA values increased significantly after frying the kamaboko and increased progressively with storage time (89.5 ± 2.07-101.8 ± 2.10 mg/g solid) compared with commercial kamaboko samples (24.8 ± 0.34-45.6 ± 0.22 mg/g solid) due to different deep frying conditions. When the food is heated by immersion in hot oil (160-195℃) as practiced in commercial frying, for a short time (2 min), the oil is not in contact with air, eliminating the possibility of oxidation and thermal alteration. In continuous industrial frying, oil is constantly being removed from the fryer with the fried food and replenished with fresh oil so that the quality of the oil remains satisfactory. This is more difficult to achieve in intermittent frying operations (DeMan, 1999).

    The TBARS confirmed the results of the TBA values, showing a similar trend (Table 3). The fried samples showed a slightly higher level (1.9-2.3 mg/g solid) than the raw samples (1.7 mg/g solid). A similar trend was observed by Oduro et al. (2011) in fried mackerel samples. A significant increase in TBARS values was observed at 60 days of storage (4.6 ± 0.52 mg/g solid). Studies on commercial kamaboko storage over 50 days showed the same trend (0, 10, 20, 30, 40, and 50 days: 1.93 ± 0.03, 2.06 ± 0.0, 2.08 ± 0.2, 2.41 ± 0.41. 3.97 ± 0.36 and 4.38 ± 0.47 mg/g solid, respectively). The increased rancidity, indicated by the TBA index is due chiefly to the formation of malonaldehyde by hydroperoxides from fatty acids containing three or more double bonds (Marti de Castro et al., 1997).

    Changes in fat oxidation determinants, TBA value and TBARS indicate a considerable increase in fat oxidation degree in the fried kamaboko products during storage under frozen conditions. Freezing slows enzymatic activity and inhibits microorganism growth, but cannot top lipid oxidation. Long-term storage will release free fatty acids from lipid/protein reactions, which will form oxidized products and cause denatured protein, which toughens. The nutritional value of proteins is lowered by reactions with peroxides, which also destroy pigments, bleach foods, produce toxins and or carcinogens, and cause off-flavors and odors. Vacuum packaging reduces autooxidation significantly (Pigott and Tucker, 1990).

      >  In vitro protein quality

    Amino acid profile

    The raw anchovy amino acid profile is similar to that reported by the NFRDI (2009). Glutamic acid had the highest concentration in all kamaboko samples (Table 4). This high concentration may also be attributable to added MSG in kamaboko products. The histidine level decreased gradually, through dissolution and removal during washing in surimi processing.

    Cysteine and tryptophan showed the lowest values in all samples. Decreased amino acid availability is the major and most serious reason for nutritive loss associated with nonenzymatic browning. Lysine is the most readily damaged essential amino acid (EAA) and becomes biologically unavailable. The loss of sulfur-containing amino acids in meat and fish can be substantial. Effects of feeding heat-damaged protein include lowered protein efficiency ratios because essential amino acids become biounavailable. Many compounds formed during the frying and barbecuing meats and fish are products of the Maillard reaction (Pigott and Tucker, 1990).

    Protein digestibility

    Three enzymes were used to determine the in vitro digestibility of samples and the reference protein casein. The in vitro methods for assaying digestibility all rely on the use of proteolytic enzymes to correlate with the digestion of protein in vivo. Generally, in vivo (rat) protein digestibility for raw fish ranges from 90.6% to 96.6% (El and Kavas, 1996).

    Table 5 shows the in vitro protein digestibility and levels of TI in raw anchovy, minced, surimi and kamaboko.

    Whole and minced anchovy had almost the same values (79.2 ± 1.01 and 79.4 ± 0.53%, respectively). Similar results (78.50%) were observed by Lee et al. (1984) and Lee and Ryu

    (1986) during the processing and storage of boiled and dried anchovy, and in the evaluation of seafood protein quality as predicted by C-PER assays, for the same species in raw samples. An increase occurred in the degree of digestibility of the surimi (84.8 ± 0.16) and kamaboko samples compared with the raw samples, but the protein digestibility of kamaboko was decreased slightly during frozen storage time. The value of digestibility at 0 days (88.5 ± 0.51%) was similar to that reported for marketed steamed kamaboko and crab meat analog (86.4-88.2%) by Ryu et al. (1994b). Protein digestibility in fried commercial kamaboko ranged from 80.9% to 91.9%. Mauron (1984), as cited by El and Kavas (1996) reported that protein digestibility was reduced as a result of complex chemical (cross-linking) reactions, such as protein interactions or protein fat interactions when food was broiled at high temperatures.

    In vitro protein quality

    The percentage of protein digestibility and C-PER of the kamaboko samples are shown in Table 6. The C-PER assay is an in vitro assay that has been developed to predict or estimate the PER of food proteins. Using a computer, a model was developed that matched the in vitro digestibility and EAA profile of the samples for respective rat-based PERs. This model is termed the “C-PER” (Pellet and Young, 1980). C-PER for kamaboko was highest (2.59) versus whole anchovy (1.96), minced (1.94), and surimi (2.50). Values of C-PER ranging from 1.97 to 2.63 were reported for commercial kamboko; values ranging from 2.32 to 2.56 for surimi and from 2.13 to 3.13 for steamed kamaboko were reported by Ryu et al (1994a, 1994b).

    Trypsin inhibitors

    Table 5 shows the changes in TI in anchovy kamaboko during frozen storage (-20℃ over 60 days). The amount of TI was highest in raw anchovy (2.43 ± 0.01 mg/g solid). In minced and surimi, the TI value decreased, probably due to lipid extraction (1.83 ± 0.07 and 0.88 ± 0.06 mg/g solid, respectively). Lee and Ryu (1986) reported that in several instances, the boiling and salting processes were thought to have resulted in

    lipid extraction, which diminished the formation of enzymeindigestible substrate. In the kamaboko samples, TI decreased gradually during storage time (0.77 to 0.31 mg/g solid) (Table 5), probably due to oxidation, as confirmed by the high TBA values and TBARS. A similar trend was observed by Oduro et al. (2011) in fried, grilled, and steamed mackerel. TI includes typical proteinaceous inhibitory materials contained in raw sources and indigestible materials such as trypsin indigestible substrates induced from the result of interactions between proteins and other components such as lipid oxidation products (Oduro et al., 2011).

      >  Starch digestibility

    Table 7 shows the values and changes in IVSD of kamaboko samples during storage (-20℃). The degree starch digestibility decreased lightly with storage time, from 14.2% to 12.5%, probably due to lipid oxidation.

    Values for commercial kamaboko ranged from 15.4% to 17.5 %. Measurement of different starch fractions provides a means of predicting the rate and extent of digestion in the human small intestine. Cooking increases the susceptibility of starches to hydrolysis; wide variation in the level of starch modification exists, depending on the processing intensity and storage conditions. The most important parameters are the water content and the maximum temperature reached. Heat increases nutrient availability. Processing may also reduce the nutritional value, as a result of losses and changes in major nutrients (Ahmed and Urooj, 2008).

      >  Texture properties

    Table 8 shows that the hardness, springiness, gumminess, and chewiness of fresh anchovy kamaboko (FAK) had non-significant differences compared with commercial kamaboko (CK), but showed significant differences with frozen and thawed kamaboko (FTAK), which showed higher values for all of these textural properties, probably because frozen storage caused structural damage to the texture. Marti de Castro et al. (1997) observed that the compression parameters (hardness, elasticity, and cohesiveness) of the frozen product were influenced mainly by freezing temperature. FAK and FTAK showed similar fracturability and adhesiveness, and difference with CK, which showed low values for fracturability. FTAK and CK showed non-significant difference in cohesiveness and resilience and significant difference in FAK, which showed the lowest values for both rheological parameters. According to Lanier (1986), as cited by Marti de Castro et al. (1997), and Gomez-Guillon and Montero (1996) cohesiveness is the most sensitive parameter for evaluating the state of surimi proteins.

    Moreover, cohesiveness and hardness can vary independently (Gomez-Guillon and Montero, 1996). According to Uresti et al. (2004), cohesiveness and chewiness show similar behaviors to hardness.

      >  Color values

    The lightness (L) value for anchovy kamaboko was lower than that of commercial kamaboko samples (Table 9). Nonsignificant differences with CK1 in lightness were noted, probably because this type of kamaboko contained vegetables, but significant differences were observed compared with samples CK2 and CK3 (without vegetables).

    The lower redness (a) value for anchovy kamaboko showed a significant difference compared with all commercial kamaboko samples, probably due the natural dark color of surimi, because anchovy is a relatively dark-fleshed fish. The heme pigments are much less readily extracted from dark fleshed species because the dark muscle structure is tougher and more rigid than white muscle (Hsu and Chiang, 2002) because myoglobin contributes to the color of the muscle (Chen et al., 1997). The increase in lightness (L) and decrease in redness (a) of washed mince are related to increased transparency as well as loss of myoglobin. The color remaining after washing was the result of insoluble pigments. Structures such as mitochondria, which contain cytochromes, would not be removed from intact muscle fragments by washing with water (Chen et al., 1997). Also, the amount of fat in the kamaboko product and unsaturated fatty acids may influence lipid oxidation, which decreases the redness.

    Nonsignificant differences in yellowness (b) value were

    observed between anchovy kamaboko and some commercial kamaboko (CK2 and CK3) samples, but a significant difference was observed with CK1, which showed the highest value of the samples.

    Decreases in lightness and increases in redness and yellowness were observed by Koo et al. (2001), Ha et al. (2001), Son et al. (2003), and Kim et al. (2003) due to the addition of increasing percentages of mushroom in the fish paste. Bae and Lee (2007) observed that increasing the amount of small and large sized anchovy powder resulted in decreases in redness and increases in yellowness. Hsu and Chiang (2002) observed that the yellowness decreased with increasing moisture content, which meant that the higher the moisture content, the whiter and the yellower the color. They reported that the increase in the lightness was not species dependent, while the decrease in the yellowness tended to be species dependent. The addition of protein additives to improve the textural properties of dark-fleshed fish species could further worsen their color properties; for example, the use of whey protein, beef plasma protein and soybean protein could increase the yellowness of the final product (Park, 1994). Color and texture properties are important sensory attributes affecting the market acceptance of surimi products. Generally, gels with high lightness, low yellowness and high whiteness are demanded by consumers (Hsu and Chiang, 2002).

  • 1. Ahmed F, Urooj A 2008 In vitro starch digestibility characteristic of Dioscorea alata Tuber. [World J Dairy Food Sci] Vol.3 P.29-33 google
  • 2. 1982 Calculated protein efficiency ratio (C-PER and DC-PER), official first action. [J Assoc Off Anal Chem.] Vol.65 P.496-499 google
  • 3. 1990 Official Method of Anaylsis. google
  • 4. Bae MS, Lee SC 2007 Quality characteristics of fried fish paste containing anchovy powder. [J Korean Soc Food Sci Nutr] Vol.36 P.1188-1192 google
  • 5. Chen HH, Chiu EM, Huang JR 1997 Color and gel-forming properties of horse mackerel (Trachurus japonicus) as related to washing conditions. [J Food Sci] Vol.62 P.985-991 google
  • 6. DeMan JM 1999 Principles of Food Chemistry. google
  • 7. El SN, Kavas A 1996 Determination of protein quality of rainbow trout (Salmo irideus) by in vitro protein digestibility: corrected amino acid score (PDCAAS). [Food Chem] Vol.55 P.221-223 google
  • 8. Gauthier SF, Vachon C, Jones JD, Savoie L 1982 Assessment of protein digestibility by in vitro enzymatic hydrolysis with simultaneous dialysis. [J Nutr] Vol.112 P.1718-1725 google
  • 9. Gomez-Guillon MC, Montero P 1996 Addition of hydrocolloids and non-muscle proteins to sardine (Sardina pilchardus) mince gels effect of salt concentration. [Food Chem] Vol.56 P.421-427 google
  • 10. Ha JU, Koo SG, Lee HY, Hwang YM, Lee SC 2001 Physical properties of fish paste containing Agaricus bisporus. [Korean J Food Sci Technol] Vol.33 P.451-454 google
  • 11. Hsu CK, Chiang BH 2002 Effect of water, oil, starch, calcium carbonate and titanium dioxide on the colour and texture of threadfin and hairtail surimi gels. [Int J Food Sci Technol] Vol.37 P.387-393 google
  • 12. Hugli TE, Moore S 1972 Determination of the tryptophan content of proteins by ion exchange chromatography of alkaline hydrolysates. [J Biol Chem] Vol.247 P.2828-2834 google
  • 13. Huss HH 1995 Quality and Quality Changes in Fresh Fish. google
  • 14. Kim SY, Son MH, Ha JU, Lee SC 2003 Preparation and characterization of fried surimi gel containing king oyster mushroom (Pleurotus eryngii). [J Kor Soc Food Sci Nutr] Vol.32 P.855-858 google
  • 15. Koo SG, Ryu YK, Hwang YM, Ha JU, Lee SC 2001 Quality properties of fish meat paste containing enoki mushroom (Flammulina velutipes). [J Kor Soc Food Sci Nutr] Vol.30 P.288-291 google
  • 16. Korczak J, He? M, Gramza A, Jedrusek Golinska A 2004 Influence of fat oxidation on the stability of lysine and protein digestibility in frozen meat products. [Electron J Pol Agric Univ] Vol.7 P.2 google
  • 17. Lee KH, Ryu HS 1986 Evaluation of seafood protein quality as predicted by C-PER assay. In: Seafood Quality Determination: Proceedings of the International Symposium on Seafood Quality Determination. Kramer DE and Liston J, eds. P.473-485 google
  • 18. Lee KH, Jo JH, Ryu HS 1984 Distribution of trypsin indigestible substrate (TI) in seafoods and its changes during processing. 2. Changes in TI and in vitro apparent digestibility of boiled and dried anchovy during processing and storage. [Bull Kor Fish Soc] Vol.17 P.101-108 google
  • 19. Marti de Castro MA, Gomez-Guillon MC, Montero P 1997 Influence of frozen storage on textural properties of sardine (Sardina pilchardus) mince gels. [Food Chem] Vol.60 P.85-93 google
  • 20. Maza Ramirez S, Llave Perez YA 2006 Mejoramiento del color de pulpa y surimi de anchoveta peruana (Engraulis ringens). [Bol Invest Inst Tecnol Pesq Peru] Vol.7 P.1-10 google
  • 21. Maza Ramirez S, Solari A 2006 Influencia del tiempo, temperatura y operaciones de refinacion en la textura del surimi de anchoveta peruana (Engraulis ringens). [Bol Invest Inst Tecnol Pesq Peru] Vol.7 P.11-19 google
  • 22. 2009 Chemical Composition of Marine Products in Korea. P.38-39 google
  • 23. Oduro FA, Choi ND, Ryu HS 2011 Effects of cooking conditions on the protein quality of chub mackerel Scomber japonicus. [Fish Aquat Sci] Vol.14 P.257-265 google
  • 24. Park JW 1994 Functional protein additives in surimi gels. [J Food Sci] Vol.59 P.525-527 google
  • 25. Pellet PL, Young VR 1980 Nutritional Evaluation of Protein Foods. P.100-101 google
  • 26. Perez AG, Guibert LN, Arana MIT, Espinoza HR, Vega AG 2010 Anuario Estadistico 2010. [Ministerio de la Produccion, Telefonos, CL] Vol.CL P.19-25 google
  • 27. Pigott GM, Tucker RW 1990 Seafood: Effect of Technology on Nutrition. P.362 google
  • 28. 1991 Food Composition Table. google
  • 29. Rhinehart D. 1975 A nutritional characterization of the distiller’s grain protein concentrates. P.29 google
  • 30. Ryu HS, Lee KH 1985 Effect of heat treatment on the in vitro protein digestibility and trypsin indigestible substrate (TIS) contents in some seafoods. [J Kor Soc Food Nutr] Vol.14 P.1-12 google
  • 31. Ryu HS, Lee KW, Lee KH 1994a Effect of processing conditions on the nutritional quality of seafood. 2. Effects of cryoprotectants on the protein qualities of Pollock surimi. [Bull Kor Fish Soc] Vol.27 P.335-343 google
  • 32. Ryu HS, Moon JH, Park JH 1994b Effect of processing conditions on the nutritional quality of seafood. 1. Effects of heating and storage conditions on protein quality of surimi products. [Bull Kor Fish Soc] Vol.27 P.282-291 google
  • 33. Son MH, Kim SY, Ha JU, Lee SC 2003 Texture properties of surimi gel containing shiitake mushroom (Lentinus edodes). [J Kor Soc Food Sci Nutr] Vol.32 P.859-863 google
  • 34. Suzuki T 1981 Fish and Krill Protein: Processing Technology. google
  • 35. Tarladgis BG, Watts BM, Younathan MT, Dugan L Jr 1960 A distillation method for the quantitative determination of melonaldehyde in rancid foods. [J Am Oil Chem Soc] Vol.37 P.44-48 google
  • 36. Uresti RM, Velazquez G, Ramirez JA, Vazquez M, Torres JA 2004 Effect of high pressure treatments on mechanical and functional properties of restructured products from arrowtooth flounder (Atheresthes stomias). [J Sci Food Agric] Vol.84 P.1741-1749 google
  • 37. Witte VC, Krause GF, Bailey ME 1970 A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. [J Food Sci] Vol.35 P.582-585 google
  • [Table 1.] Proximate composition of raw anchovy, minced, surimi and kamaboko
    Proximate composition of raw anchovy, minced, surimi and kamaboko
  • [Table 2.] Changes in water activity of raw anchovy, minced, surimi and kamaboko in frozen storage (-20℃)
    Changes in water activity of raw anchovy, minced, surimi and kamaboko in frozen storage (-20℃)
  • [Table 3.] TBA value and TBARs of raw anchovy, minced, surimi and kamaboko in frozen storage at -20℃
    TBA value and TBARs of raw anchovy, minced, surimi and kamaboko in frozen storage at -20℃
  • [Table 4.] Amino acid profiles of raw anchovy, minced, surimi and kamaboko
    Amino acid profiles of raw anchovy, minced, surimi and kamaboko
  • [Table 5.] In vitro protein digestibility and level of trypsin inhibitor of raw anchovy, minced, surimi and kamaboko
    In vitro protein digestibility and level of trypsin inhibitor of raw anchovy, minced, surimi and kamaboko
  • [Table 6.] In vitro protein qualities of raw anchovy, minced, surimi and kamaboko
    In vitro protein qualities of raw anchovy, minced, surimi and kamaboko
  • [Table 7.] In vitro starch digestibility of raw anchovy, minced, surimi and kamaboko
    In vitro starch digestibility of raw anchovy, minced, surimi and kamaboko
  • [Table 8.] Texture properties for fresh fried, frozen and thawed anchovy kamaboko and commercial kamaboko
    Texture properties for fresh fried, frozen and thawed anchovy kamaboko and commercial kamaboko
  • [Table 9.] Color value for frozen anchovy kamaboko and different types of commercial kamaboko
    Color value for frozen anchovy kamaboko and different types of commercial kamaboko