A marine algal species was collected from the southern coast of Korea. This alga is characterized by a large and cartilaginous thallus, a dichotomous branching pattern, compressed axes, rare proliferations near the apex, and cystocarps protruded toward surface. In a phylogenetic tree based on rbcL sequences, this species nests in the same clade as Neodilsea yendoana as a terminal clade of the genus Neodilsea. The genetic distance between both sequences within the clade was calculated as 0.2% considered to be in the intraspecific range within the genus. Based on the morphological and molecular data obtained in the present study, this Korean species is identified as N. yendoana. This is the first record of the genus Neodilsea and N. yendoana in Korea.
A red algal species belonging to the Dumontiaceae was collected from the southern coast of Korea during a survey of indigenous species of marine algae. This species is identified as
Specimens for this study were collected along the southern coast of Korea. Taxonomic data were obtained from fresh, liquid-preserved and herbarium specimens. Liquid-preserved material was stored in a 10% solution of formalin/seawater. For anatomical observations, the material was cleared in 5–10% NaOH in distilled water for 2–7 days, and then rinsed in distilled water. Blades dissected from the cleared materials were hand sectioned, transferred to a slide with a drop of distilled water, and mounted in pure glycerin. In some instances, a smearing method for microscopic examination was employed. Measurements are given as widths and lengths. For photographs, the sections were stained with 0.5–1.0% aqueous methylene blue, aniline blue, or hematoxylin. For permanent slides, the glycerin was exchanged with 10–20% corn syrup.
Total genomic DNA was extracted from silica gel-dried samples using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. Before extraction, dried material was crushed with liquid nitrogen using a mortar and pestle. Concentrations of extracted DNA were assessed by using gel electrophoresis on a 1% agarose gel. Extracted DNA was used for the amplification of ribulose-1, 5-bisphosphate carboxylase large subunit (
Sequences for the
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Neodilsea yendoana
Type locality: Osyoro, Prov. Otaru, Hokkaido, Japan.
Specimens examined: NIBR 0000146289, PKNU 0000 127055, PKNU 0000127048, PKNU 0000127058, PKNU 0000127060 (Jindo: 13.ii.2014).
Habitat: Rock near upper to lower intertidal zone.
Morphology: The thalli are 10–15 cm high (Fig. 1a), flattened, multiaxial, red to brown in color, gelatinous in texture, and attached on the substrate by discoid holdfast. The main axes have dichotomous branches. The branches are divided dichotomously to subdichotomously (Fig. 1b), with a rounded or blunt apex, and an entire margin that is 1–2 cm wide and 300–400 μm thick. The proliferations are rare (Fig. 2a) and arranged pinnately to irregularly. The axial cells are large (Fig. 2d). The cortex consists of small and pigmented cells (Fig. 2b) and is one to two cell layers thick (4–5 × 5–6 μm) (Fig. 2c). The medullary cells are arranged in one to three layers and are ellipsoid (100–150 × 50–100 μm) in the transverse section. In female plant, the supporting cell is intercalarily large and ellipsoidal (Fig. 3a). The carpogonial branch is produced from the large supporting cell (Fig. 3b). The gonimoblast initial is produced from auxiliary cell transformed from supporting cell (Fig. 3c). The gonimoblast filaments develop inwardly toward medulla (Fig. 3d), and then form carposporophytes with masses of carposporangia. The carposporangia are round and 10–12 μm in diameter. The cystocarps are immersed in the medulla solitarily or in irregular groups, but are protruded toward surface. They have a thickened pericarp (Fig. 3e and 3f). Male and tetrasporangial plants were not collected during the present study.
In a phylogenetic tree based on the
