The family Sillaginidae, order Perciformes, comprise 31 species in three genera worldwide (Nelson, 2006) and 4 species in 1 genus in Korea (Kim et al., 2005; Kwun and Kim, 2010). The Korean species are
A single specimen of
Genomic DNA was extracted from muscle tissue using Chelex 100 resin (Bio-Rad, Hercules, CA, USA). Polymerase chain reaction (PCR) was used to amplify the mitochondrial DNA cytochrome oxidase subunit I (COI) using universal primer set (VF2: 5′-TCAACCAACCACAAAGACATTGG CAC-3′ and FishR1: 5′-TACACTTCTGGGTGGCCAAA GAATCA-3′) (Ivanova et al., 2007), with PCR solution containing 5 μL of genomic DNA, 5 μL of 10× buffer, 4 μL of 2.5 mM dNTPs, 1 μL of each primer, 0.5 μL of FR Taq polymerase (Biomedic, Korea), and distilled water to bring the final volume to 50 μL. PCR was performed under the following conditions: initial denaturation was for 2 min at 95℃, followed by 35 cycles of 30 s at 94℃ for denaturation, 30 s at 61℃ for annealing, and 30 s at 72℃ for extension, with a final extension at 72℃ for 3 min. The nucleotide sequence was deposited in the DDBJ/EMBL/GenBank databases (accession no. KC708229). The sequence was aligned with ClustalW (Thompson et al., 1994) in BioEdit version 7 (Hall, 1999). The sequences of 4
[Table 1.] Comparison of meristics and morphometrics of Sillago sinica and S. parvisquamis
Comparison of meristics and morphometrics of Sillago sinica and S. parvisquamis
(NJ) tree was constructed in MEGA 5 (Tamura et al., 2011) with 1,000 bootstrap replications.
Order Perciformes
Family Sillaginidae
1*Sillago sinica Gao and Xue in Gao, Ji, Xiao, Xue, Yanagimoto and Setoguma, 2011
(new Korean name: Buk-bang-jeom-bo-ri-myeol)
(Table 1, Fig. 1)
Sillago sinica Gao and Xue in Gao, Ji, Xiao, Xue, Yanagimoto and Setoguma, 2011: 256, figs. 1, 3F (type locality: coastal waters of East China Sea, China).
Sillago parvisquamis (non Gill): Kwun and Kim, 2010: 109, figs. 2B, 3B (Korea).
Material examined. NIBR-P0000019930 (previously PKU2043), 1 specimen, 157.0 mm in standard length (SL),
Gwangyang, Korea, 29 May 2009; collected by Kwun HJ and Kim JK.
Description. Counts and measurements are shown in Table 1. Body elongated, slightly compressed, and head tapering. Mouth small, terminal, and snout long. Body depth low, and dorsal margin of head slightly sloping. Eyes normal and located middle of the head, and cheek large. Posterior tip of maxilla not reaching to anterior margin of eye, and upper jaw projecting beyond lower jaw. Single row of conical teeth on both jaws. Two pairs of nostrils located in front of eyes. Interorbital region flat and covered with scales. Lateral line extending to caudal fin base with a slight curvature along middle of body. Body and head covered with ctenoid scales; cheek covered with both ctenoid and cycloid scales; only small scales on base of caudal fin. Posterior margin of preopercle serrated. Opercle with a small spine. Two dorsal fins that appear contiguous but completely separated; origin of first dorsal fin located posterior to origin of pelvic fin; origin of second dorsal fin located vertically above origin of anal fin. Second dorsal spine longest, and others gradually became shorter. Pectoral fin slender. Origin of pelvic fin located vertically above lowest base of pectoral fin, and pelvic fin rays shorter than pectoral fin rays. Caudal fin slightly emarginate but nearly truncate.
Coloration. When fresh, body is yellowish-green dorsally and silver-white ventrally. Darkish brown band on snout, gradually fading posteriorly. Two dorsal fins with transparent membranes; first fin darkish anteriorly and second fin with 3 or 4 rows of dusky spots. Pectoral fin pale yellow; pelvic fin white; anal fin membrane transparent with small irregular black spots; caudal fin yellowish with darkish posterior lobe. After fixation, body is bright yellow and silvery white from upper base of the pectoral fin to the ventral side. A single faint stripe is present along middle of body.
MtDNA COI analysis. A total of 550 bp of mitochondrial DNA cytochrome oxidase subnit I (mtDNA COI) were obtained from our specimen and the base pair sequence was then compared with those of four Sillaginidae species. The sequence from our specimen corresponds exactly to that of
Distribution.
Remarks. The specimen in this study was identified as
Korean name: 1*북방점보리멸