The genus Strongylocentrotus of the family Strongylocentro-tidae consists of nine species globally (Smith, 2005; Kroh and Mooi, 2011): S. djakonovi, S. droebachiensis, S. franci-scanus, S. intermedius, S. nudus, S. pallidus, S. polyacan-thus, S. pulchellus and S. purpuratus. Among them, S. inter-medius and S. nudus were reported in Korea (Shin and Rho,1996; Shin, 1998, 2011). The former inhabits only the coast of East Sea but the latter inhabits all coastlines of South Korea except Jeju-do Island (Shin, 2011). Some echinoids were collected from Daejin to Imwon harbors in Gangwon-do and were identified. Among them, S. pallidus is newly re-ported in Korea. Mortensen (1943) reported that morphologi-cal characteristics of S. pallidus were not distinctly distin-guished from the adjacent species such as S. echinoides and S. sachalinicus. Recently, these two species were recorded as synonymous with S. pallidus by morphological and mole-cular evidences (Jensen, 1974; Tatarenko and Poltarous, 1992; Bazhin, 1998). Therefore, we examined thoroughly morphological characteristics of Korean Strongylocentrotus species and analyzed the molecular differences between Ko-rean species and Genbank data of S. pallidus and then other adjacent species using cytochrome oxidase subunit I (COI) mitochondrial DNA (mtDNA).

The specimens of Strongylocentrotus were collected using fishing nets at depths of 50-190 m from nine coastal areas in Gangwon-do from November 2008 to July 2011 (Table 1). Specimens were preserved in 95% methyl alcohol, and their important morphological characters were photographed by light- and stereo-microscopes (Nikon Eclipse 80i, Nikon SMZ1000; Nikon Co., Tokyo, Japan). Identification of spe-cimens referred to Mortensen (1943), Southward and Camp-bell (2006) and Shin (2011).

Genomic DNA was extracted from the gonad tissues of echinoids using by DNeasy blood and tissues kit (Qiagen, Hilden, Germany) and the COI gene was amplified using primers of Knott and Wray (2000): ECO1a (5′-ACCATGC AACTAAGACGATGA-3′) and ECO1b (5′-GGTAGTCTGAGTATCGTCGWG-3′). PCR amplification chemistry con-taining 1.5 μL of genomic DNA, 2.5 μL of 10× PCR buffer (contained MgCl2), 1.0 μL of 2.0 mM dNTPs and each pri-mer, 0.3 μL of nTaq DNA polymerase (Enzynomics, Seoul, Korea) and add up to 25.0 μL with distilled water, and the following conditions: initial denaturation of 2 min at 95℃, 30 cycles of 95℃ 30 sec; 52℃ 1 min; and 72℃ 1 min and a final elongation of 7 min at 72℃. DNA fragments were sequ-enced on an ABI 3730XL sequencer (Applied Biosystems Inc., Forster City, CA, USA) using the ABI Prism Bigdye Terminator v3.1 (Applied Biosystems Inc.).

The mitochondrial COI gene was mostly sequenced for this study, but the sequences data of four species which are not distributed in Korea obtained from GenBank (Table 2). COI

sequences were checked and aligned using by BioEdit v.7.0 (Hall, 1999) and genetic distances were calculated accord-ing to the Kimura 2-parameter model (Kimura, 1980) using by MEGA5 (Tamura et al., 2011). The phylogenetic rela-tionship of the samples was drawn by using the neighbor-joining (NJ), maximum-likelihood (ML) and Bayesian infer-ence (BI). The NJ tree (Saitou and Nei, 1987) was inferred from Kimura 2-parameter genetic distance with bootstrapp-ed 1,000 times using by MEGA5, and the ML analysis with the GTR+G model, determined with jModeltest 0.1.1 (Guin-don and Gascuel, 2003; Posada, 2008), with 1,000 bootstrap replications using by PhyML v3.0 (Guindon and Gascuel, 2003) also BI analysis with same model and analyzed by MrBayes 3.1 with 1×10⁶ generation repeats with the nu-cleotide model 4by4, Nst=6, rates=gamma, Ngammacat=6, and Burnin=2.5×10⁵ (Huelsenbeck and Ronquist, 2001; Ronquist and Huelsenbeck, 2003; Ronquist et al., 2005).

Korean name: 1*연약둥근성게 (신칭)

Class Echinoidea Leske, 1778

Subclass Euechinoidea Bronn, 1860

Order Camarodonta Jackson, 1912

Infraorder Echinidea Kroh and Smith, 2010

Family Strongylocentrotidae Gregory, 1900

Genus Strongylocentrotus Brandt, 1835

^1*Strongylocentrotus pallidus (Sars, 1871)

Toxopneustes pallidus Sars, 1871: 25.

Strongylocentrotus pallidus Bidenkap, 1899: 112; Jensen, 1974: 119; Vader et al., 1986: 10; Southward and Camp-bell, 2006: 144; Kroh and Mooi, 2011: 124324.

Strongylocentrotus drøbachiensis var. sachalinicus Doderl-ein, 1906: 517.

Strongylocentrotus echinoides A Agassiz and HL Clark, 1907: 122; HL Clark, 1912: 360; Mortensen, 1943: 219; Downey, 1968: 82.

Strongylocentrotus sachalinicus HL Clark, 1912: 353; Mor-tensen, 1943: 215; Kroh and Mooi, 2011: 513826.

Strongylocentrotus drøbachiensis sachalinica D’yakonov, 1938: 470, 496.

Key to the species of genus Strongylocentrotus in Korea

1. Ambulacral pore-pairs five in number ？？？？？？ S. intermedius Ambulacral pore-pairs six in number ？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？ 2

2. Primary spines long and stout ？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？？ S. nudus Primary spines short and not stout ？？？？？？？？？？？？？？？？？？？？？ S. pallidus

Description.Test form vertically very variable (Table 3); flattened (Fig.1 G), low-hemispherical (Fig.1F, H) and hemi-spherical forms (Fig.1 I). Outline of test roundly pentagonal forms (Fig.1 D). Margin of oral side slightly sunken towards peristome. Test of largest specimen 59 mm in diameter. Six ambulacral pore-pairs presented in an erected arc (Fig.1 K-M). Madreporite has convexed form, larger than genital plates (Fig.1 J). Ocular plates composed five plates, three plates has pentagonal form, stuck between genital plates, like wedges, the other two plates have hexagonal form, situ-ated between genital plates, bordering surnal parts. Surnal parts consist of numerous small plates, with anus situated beside center, surrounded with small blunt spines (Fig.1 J). Globiferous pedicellaria has single slender apical tooth, without lateral tooth (Fig.1 O). Tridentate pedicellaria has two different sizes, large and small form; large ones about five times larger than small one (Fig.1 P, Q). Ophiocephal-ous pedicellaria with fountain pore-pattern valves (Fig.1 R). Triphyllous pedicellaria apple-like shaped, with upraised radial pore-pattern (Fig.1 S). Spicule of tube-foot usually elongated arch form, rarely twisted form, which has trifur-cated end, of which inside node slender, tapered to tip (Fig.1T).

Distribution. Korea (Gangwon-do), Japan (Siaukhu Bay), Bering Sea, Okhotsk Sea, North Pacific (Kuril island, Sakah-lin), Northwest Atlantic (Norway, U.K.).

DNA sequence features. A total of 1,214 base pairs (bp) of the COI mtDNA were obtained from five specimens of Strongylocentrotus pallidus and the other Korean echinoids such as S. intermedius, S. nudus and Heliocidaris crassis-pina (Table 2). But, GenBank sequences were shorter than our sequences and so 818 bp COI mtDNA were analyzed in this study. In a group of S. pallidus, only 4 bp of the 818 bp was different from each.

Phylogenetic tree. The phylogenetic relationships of the COI mtDNA from Strongylocentrotus species were analyz-ed by BI, ML and NJ method. We appointed Heliocidaris crassispina as the outgroup, and analyzed with S. droe-bachiensis, S. pallidus, S. polyacanthus and Allocentreotus fragilis obtained from GenBank. In the phylogenetic trees, BI, ML and NJ analyses represented the non-discrimenatory phylogenetic branches (Figs. 2, 3). Korean specimens of S.pallidus coincident with S. pallidus of GenBank, and our phylogenetic reconstruction suggests that the genus Strongy-locentrotus with A. fragilis is paraphyletic, but A. fragilis is closely related to S. pallidus and S. droebachiensis and its taxonomical position still obscure. Therefore the genus Stron-gylocentrotus without A. fragilis is monophyletic.

Genetic distances. Kimura 2-parameter genetic distance between Strongylocentrotus pallidus (Korea) and S. pallidus

(GenBank) ranged from 0.002 to 0.005 (Table 4). Pairwise(p) distance average of the Strongylocentrotus group is 0.062 and excepted S. nudus of Strongylocentrotus group is 0.041. Average of between S. pallidus and Allocentrotus is 0.045, which was lower than average of the Strongylocentrotus group, and between S. pallidus and S. droebachiensis dis-

tance (=0.039) was lower than the Strongylocentrotus group average.

Two species of Strongylocentrotus (S. intermedius and S.nudus) were previously reported (Shin, 2011). In this study, S. pallidus is newly reported, which has distinct characteri-stics compared with two species: external figure, pedicella-riae and number of pore-pairs in an arc. Strongylocentrotus pallidus has various range of test height; the range of hei-ght/diameter of Korean specimens is 38.2-50.0% and range of peristome/diameter is 31.6-35.7% (Table 3). However, Mortensen (1943) described the range of peristome/diame-ter as 31.6-45.0%. Thus, we divided the three groups based on the test height: Group-1 (flattened form)= S. pallidus (A), Group-2 (low-hemispherical form)= S. pallidus (B)- (D) and Group-3 (hemispherical form)= S. pallidus (E). Korean speci-mens of S. pallidus revealed as identical with S. pallidus of GenBank. According to the results of phylogenetic analysis and genetic distance (Figs. 2, 3, Table 4). In the phylogene-tic tree, S. pallidus group divided by two clades (Figs. 2, 3); S. pallidus (A), (E) and S. pallidus (B)-(D). But, that is not a significant cladogram, because the intraspecific p-distance average value is only 0.003 and is much lower than other interspecific p-distances (Table 4).

Strongylocentrotus pallidus is very similar with S. droe-bachiensis and can only be discriminated by rather impal-pable differences (Vasseur, 1951; Swan, 1962; Jensen, 1974;Vader et al., 1986). For that reason, it has been included in S. droebachiensis (Mortensen, 1943). In these phylogenetic results, S. pallidus and S. droebachiensis showed rather close relationships (Table 4); p-distance value is only 0.039, which is lower than average of Strongylocentrotus (0.062) and in phylogenetic tree, S. pallidus were branched off close to S. droebachiensis. Thus, echinoid specimens are unrecord-ed species examined in Korea by determining on the basis of the morphological and molecular evidences. Recently, they were divided from each other on the basis of morphological characters within populations and between geographical districts, genetic differences and capacity for hybridization (Vasseur, 1951; Jensen, 1974; Vader et al., 1986; Biermann et al., 2003).

Mortensen (1943) reported that Allocentrotus fragilis is very unlike any of the true species of Strongylocentrotus. However, previous studies (Strathmann, 1979; Biermann et al., 2003) and our phylogenetic results (Figs. 2, 3) show rather close relationships between A. fragilis and the species of Strongylocentrotus. The taxonomical position of A. fragi-lis needs to be consider in a further study.