Diverse and polyphyletic species in the
Here, we redescribe two urostylid ciliates,
DNA extraction, amplification and sequencing of nearly complete SSU rDNA were performed according to Jung et al. (2011). Our sequences and retrieved sequences from Gen-Bank were aligned using BioEdit (Hall, 1999), and pairwise genetic distance based on Kimura two-parameter inference was obtained using MEGA 4.0 (Kumar et al., 2004).
Comparison of morphometric data from Anteholosticha pulchra with Li et al. (2007)
Korean name: 1*홍색옛전열하모충 (신칭)
Phylum Ciliophora Doflein, 1901
Class Spirotrichea Butschli, 1889
Order Urostylida Jankowski, 1979
Family Urostylidae Butschli, 1889
Genus Anteholosticha Berger, 2003
1*Anteholosticha pulchra (Kahl, 1932) Berger, 2003 (Table 1, Figs.1 A-E, 2)
[Fig. 1.] Morphology of Anteholosticha pulchra and Metaurostylopsis struederkypkeae from live (A-C F-J) and protargol impregnated (D E K L) specimens. A-E Anteholosticha pulchra: A Ventral view of live contractile vacuole (arrow); B C Pattern of cortical granules in dorsal and ventral views reddish cortical granules (arrow); D E Cirral base in ventral and dorsal views from protargol impregnation; F-L Metaurostylopsis struederkypkeae: F Ventral view of live contractile vacuole (arrow); G H Ventral and dorsal views yellow-green cortical granules (arrow) small reddish cortical granules (arrowhead); I J Ventral and dorsal views which show the arrangement of yellow-green cortical granules; K L Cirral base on ventral and dorsal views with protargol impregnation. AZM adoral zone of membranelles; BC buccal cirrus; DK dorsal kineties; EM endoral membrane; FC frontal cirri; FTC frontoterminal cirri; LMR left marginal row; Ma macronuclear nodules; MP midventral pairs; MVR midventral row; PM paroral membrane; RMRright marginal row; TC transverse cirri. Scale bars: A-E=100 μm F-L=50 μm.
[Fig. 2.] Micrographs of Anteholosticha pulchra from live (A-E) and protargol impregnated (F-L) specimens. A B Live ventral (A) anddorsal (B) view arrangement of cortical granules (arrowhead); C Ventral view contractile vacuole (arrow); D Dorsal view reddishcortical granules (arrow); E Live ventral view of transverse cirri; F G Dorsal and ventral views of protargol-impregnated specimen; H Ventral view the frontal (arrows) and frontoterminal (arrowhead) cirri; I Buccal cirri (arrow); J Ventral view the transversecirri; K Dorsal view the dorsal kineties typically four; L Macronuclear nodules (arrow). Scale bars: A B F G=100 μm.
mately 10 μm in diameter, positioned on left side of posterior 1/4 of cell (Figs.1 A, 2B, C, arrow). Spherical-reddish corti-cal granules at cirral bases and around dorsal bristles,
46-60 membranelles in adoral zone,
Distribution. Germany (Kiel, the Baltic Sea), China (Laiz-hou, the Yellow Sea), and Korea (this study).
Remarks. Polyphyly of genus
Comparison of morphometric data from Metaurostylopsis struederkypkeae with Shao et al. (2008)
ording to the relativeness of both morphology and phylogeny (Berger, 2006; Gao et al., 2010; Yi and Song, 2011). Among
The Korean population corresponds well with Chinese population reported by Li et al. (2007), and showed high sim-ilarity each other in morphometric characteristics (Table 1).
The SSU rDNA sequence of Korean
1*Genus Metaurostylopsis Song, Petz and Warren, 2001
2*Metaurostylopsis struederkypkeae Shao et al., 2008 (Table 2, Figs.1F-L, 3)
Description. Live cell size usually 80-110× 40-50 ㎛ (Figs.1F, 3A-C), slender in shape and slightly contractile, with an-terior and posterior ends rounded, and contractile vacuole approximately 10㎛ in diameter located on left side of equa-torial level of cell (Fig.3 C, arrow). Two types of cortical granules: one small and reddish, approximately 0.5㎛ across, spread irregularly on surface (Figs.1 G, H, 3D, arrowhead), and other one yellow-green, approximately 1.5 ㎛ across, situated at base of cirri, with some cortical granules clustered along cirral rows and dorsal kineties (Figs.1 G, H, 3D, arrow). Color of cells appeared reddish under low magnification due to numerous small cortical granules. Lengths of transverse and frontal cirri 15 ㎛ and 13 ㎛, respectively. Other soma-tic cirri, midventral, left and right marginal cirri,
Distribution. China (Qingdao, the Yellow Sea) and Korea (this study).
Remarks.There are six species in this genus:
The Korean population of
[Fig. 3.] Micrographs of Metaurostylopsis struederkypkeae from live (A-D) and protargol impregnated (E-H) specimen. A B Live ven-traland dorsal views; C Contractile vacuole (arrow); D Yellow-green cortical granules (arrow) small reddish cortical granules (arrow-head); E Ventral view of protargol-impregnated specimen; F Ventral view left and right marginal rows; G Dorsal view typicallythree dorsal kineties (arrows) and two dikinetids (arrowheads); H Frontoterminal (arrow) and buccal (arrowhead) cirri. Scale bars: A-C E=50 ㎛.
Kimura two-parameter pairwise distances (%) among the five species in Anteholosticha-Nothoholosticha assemblage basedon small subunit ribosomal DNA sequences
number of left and right marginal rows, midventral pairs, and macronuclear nodules than those of the Chinese population (Shao et al., 2008).
The SSU rDNA sequence of
Kimura two-parameter pairwise distance (%) based on small subunit ribosomal DNA sequences
Korean name: 1*사미주하모충 (신칭), 2* 작은홍색사미주하모충 (신칭)