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Involvement of Transient Receptor Potential Melastatin 7 Channels in Sophorae Radix-induced Apoptosis in Cancer Cells - Sophorae Radix and TRPM7 -
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ABSTRACT
Involvement of Transient Receptor Potential Melastatin 7 Channels in Sophorae Radix-induced Apoptosis in Cancer Cells - Sophorae Radix and TRPM7 -
KEYWORD
Sophorae Radix , transient receptor potential melastatin 7 (TRPM7) channel , gastric cancer , breast cancer
참고문헌
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  • [ Figure 1 ]  Sophorae Radix (SR) induces cell death in AGS and MCF-7 cells. (A) The AGS cells were incubated with SR at the indicated concentrations for 72 hrs prior to MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assays. Distilled water was used as a vehicle. Cell viability is expressed as a value relative to that of the untreated cells which is set to 100%. (B) Time course response to SR. AGS cell viability is expressed as a value relative to that of the cells treated with a vehicle and harvested at zero time which is set to 100%. (C) The MCF-7 cells were incub-ated with SR at the indicated concentrations for 72 hrs prior to MTT assays. Distilled water was used as a vehicle. Cell viability is expressed as a value relative to that of the untr-eated cells which is set to 100%. (D) Time course response to SR. MCF-7 cell viability is expressed as a value relative to that of the cells treated with a vehicle and harvested at zero time which is set to 100%. The figures show mean ± SEM. *P? 0.05, **P? 0.01.
    Sophorae Radix (SR) induces cell death in AGS and MCF-7 cells. (A) The AGS cells were incubated with SR at the indicated concentrations for 72 hrs prior to MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assays. Distilled water was used as a vehicle. Cell viability is expressed as a value relative to that of the untreated cells which is set to 100%. (B) Time course response to SR. AGS cell viability is expressed as a value relative to that of the cells treated with a vehicle and harvested at zero time which is set to 100%. (C) The MCF-7 cells were incub-ated with SR at the indicated concentrations for 72 hrs prior to MTT assays. Distilled water was used as a vehicle. Cell viability is expressed as a value relative to that of the untr-eated cells which is set to 100%. (D) Time course response to SR. MCF-7 cell viability is expressed as a value relative to that of the cells treated with a vehicle and harvested at zero time which is set to 100%. The figures show mean ± SEM. *P? 0.05, **P? 0.01.
  • [ Figure 2 ]  SR triggers apoptosis in AGS cells. (A) Mitochondria membrane depolarization is expressed as a value relative to that of untreated cells which is set to 100%. (B) The cells were cultured with SR at the indicated concentrations for 24 hrs prior to caspase assays. Caspase activity from untreated cells is expressed as 100%. Pan-caspase inhibitor zVAD-fmk (zVAD) at 20 μM was used to validate the analytical method employed. (C) A representative western blot of incubated cells with anti-poly (ADP-ribose) polymerase (PARP) antibody. Cells incubated with SR represent increased PARP cleavage. Glyceraldhyde-3- phosphate dehydrogenase (GAPDH) was used as an internal control. The figures show mean ± SEM. *P? 0.05, **P? 0.01.
    SR triggers apoptosis in AGS cells. (A) Mitochondria membrane depolarization is expressed as a value relative to that of untreated cells which is set to 100%. (B) The cells were cultured with SR at the indicated concentrations for 24 hrs prior to caspase assays. Caspase activity from untreated cells is expressed as 100%. Pan-caspase inhibitor zVAD-fmk (zVAD) at 20 μM was used to validate the analytical method employed. (C) A representative western blot of incubated cells with anti-poly (ADP-ribose) polymerase (PARP) antibody. Cells incubated with SR represent increased PARP cleavage. Glyceraldhyde-3- phosphate dehydrogenase (GAPDH) was used as an internal control. The figures show mean ± SEM. *P? 0.05, **P? 0.01.
  • [ Figure 3 ]  SR triggers apoptosis in MCF-7 cells. (A) Mitochondria membrane depolarization is expressed as a value relative to that of untreated cells which is set to 100%. (B) The cells were cultured with SR at the indicated concentrations for 24 hrs prior to caspase assays. Caspase activity from untreat-ed cells is expressed as 100%. Pan-caspase inhibitor zVAD-fmk (zVAD) at 20 μM was used to validate the analytical method employed. (C) A representative western blot of incubated cells with anti-poly (ADP-ribose) polymerase (PARP) antibody. Cells incubated with SR represent increased PARP cleavage. Glyceraldhyde-3- phosphate dehydrogenase (GAPDH) was used as an internal control. The figures show mean ± SEM. *P? 0.05, **P? 0.01.
    SR triggers apoptosis in MCF-7 cells. (A) Mitochondria membrane depolarization is expressed as a value relative to that of untreated cells which is set to 100%. (B) The cells were cultured with SR at the indicated concentrations for 24 hrs prior to caspase assays. Caspase activity from untreat-ed cells is expressed as 100%. Pan-caspase inhibitor zVAD-fmk (zVAD) at 20 μM was used to validate the analytical method employed. (C) A representative western blot of incubated cells with anti-poly (ADP-ribose) polymerase (PARP) antibody. Cells incubated with SR represent increased PARP cleavage. Glyceraldhyde-3- phosphate dehydrogenase (GAPDH) was used as an internal control. The figures show mean ± SEM. *P? 0.05, **P? 0.01.
  • [ Figure 4 ]  Inhibition of cell death by transient receptor potential melastatin (TRPM7) blockade. (A) MTT assay induced by different treatment as indicated in AGS (a) and MCF-7 (b) cells. Inhibition of TRPM7 channels by Gd3 or 2-APB reduced SR induced apoptosis. (B) Effect of SR on TRPM7-like current in AGS cells. I-V curves (a) and summary bar graph (b) in the absence (■) or presence (●) of SR. (C) Effect of SR on the TRPM7-like current in MCF-7 cells. I-V curves (a) and summary bar graph (b) in the absence (■) or presence (●) of SR. **P ? 0.01.
    Inhibition of cell death by transient receptor potential melastatin (TRPM7) blockade. (A) MTT assay induced by different treatment as indicated in AGS (a) and MCF-7 (b) cells. Inhibition of TRPM7 channels by Gd3  or 2-APB reduced SR induced apoptosis. (B) Effect of SR on TRPM7-like current in AGS cells. I-V curves (a) and summary bar graph (b) in the absence (■) or presence (●) of SR. (C) Effect of SR on the TRPM7-like current in MCF-7 cells. I-V curves (a) and summary bar graph (b) in the absence (■) or presence (●) of SR. **P ? 0.01.
  • [ Figure 5 ]  Effects of RNA interference (RNAi) in AGS and MCF-7 cells and the effect of SR on transient receptor potential melastatin 7 (TRPM7) channel overexpression in human embryonic kidney (HEK) cells. (A) AGS cell viability was increased 72 hrs after transfection with TRPM7siRNA and incubation with SR. (B) MCF-7 cell viability was increased 72 hrs after transfection with TRPM7siRNA and incubation with SR. (C) TRPM7 cells were treated or not treated with tetracycline for 1 day. Cells were incubated with SR, followed by MTT assay. **P? 0.01.
    Effects of RNA interference (RNAi) in AGS and MCF-7 cells and the effect of SR on transient receptor potential melastatin 7 (TRPM7) channel overexpression in human embryonic kidney (HEK) cells. (A) AGS cell viability was increased 72 hrs after transfection with TRPM7siRNA and incubation with SR. (B) MCF-7 cell viability was increased 72 hrs after transfection with TRPM7siRNA and incubation with SR. (C) TRPM7 cells were treated or not treated with tetracycline for 1 day. Cells were incubated with SR, followed by MTT assay. **P? 0.01.
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