Ascidians comprise one of four classes belonging to the subphylum Tunicata of the phylum Chordata and are commonly called sea squirts or tunicates. All ascidians are filter feeders and are either solitary or colonial, and their individual forms called zooids, are often arranged in systems around a common cloacal opening. The class Ascidiacea includes three order, Aplusobranchia, Phlebobranchia, and Stolidobranchia (Kott, 1992). Aplusobranchia is represented by a colonial form, and Stolidobranchia and Phlebobranchia mainly by solitary species (Shirley, 2003). Zooids of Aplousobranchia have their gut loop below their branchial sac. The structure of the branchial sac, the gut, and the position and structure of the gonads are all important characteristics in identifying the various genera and species (Kott, 1992). Aplousobranchia consists of 13 families, including Clavelinidae. Genus
Phylum Chordata Bateson, 1885
Subphylum Tunicata Lamarck, 1816
Class Ascidiacea Nielsen, 1995
Order Aplousobranchia Lahille, 1886
Family Clavelinidae Forbes and Hanley, 1848
Genus
1*Clavelina lepadiformis (Muller, 1776) (Fig. 1A-G)
Materials examined. 32 zooids, Seogwipo, 15 Aug 2008, Shin S, attached to ropes at 2.3 m depth; 21 zooids, Daebyeon, 14 Aug 2009, Shin S, attached to dock wall at 2.5m depth; 202 zooids, Gampo, 24 Jun 2010, Lee T, attached to ropes at 2 m depth; 124 zooids, Bangeojin, 25 Jun 2010, Shin S, attached to bivalve (
Description. Zooids 15-42 mm long×2.0-4.7 mm diameter.Colony elongated, living in groups of dozens to hundreds of zooids attached to base by stolons. Zooid transparent but marked with pale yellow or white lines comprising small white rings around two siphons and large conspicuous white ring around pharynx at top of brachial sac and two longitudinal lines along endostyle and dorsal lamina, as well as white ring around lowest part of thorax. Additional white pigments usually on edge of oral siphon which more apparent than that of atrial siphon. Both siphons rounded orifices not far from each other, with oral siphon larger than atrial one. Tunic perfectly transparent, colorless, but usually marked with opaque white lines, through which internal organ visible to naked eye. Thorax corresponding to about a third of entire length,sometimes nearly one half in life. A few widely separated longitudinal fibers observed in thoracic portion. Mantle diaphanous, very delicate, and torn easily. Branchial sac nearly fills thoracic portion, with 14 or 15 rows of about equal sized meshes placed at regular intervals, making branchial membrane form simple rectangular network with narrow and longer meshes. Endostyle well developed, opaque white color, extended whole length of branchial sac, and almost met with margin of oral siphon. Stomach looks like orange bilobed mass with white lines, round but sometimes elongated and considerably wider than rest of alimentary tract, hanging from esophagus, and occupying half way down peduncle. Intestine running along lower end of stomach, continuing downwards and then immediately upwards to anus. Gonads rather conspicuous and located between loops of intestine near posterior part of abdominal cavity. Ovary forms like a bunch of grapes, and many various sized eggs usually found near anus. Testis forms a much-branched organ, with extremely fine ramifications terminating in numerous elongated vesicles usually bifurcated or rarely trifurcated, spread around ovary, and gonoduct passed up along right side of intestine and terminated by side of anus.
Distribution. Korea (Korea Strait, Jejudo Island), North East Atlantic (England, Scotland, Norway-Mediterranean), North West Atlantic (Connecticut), and South Africa.
Remarks.This species is a colonial tunicate commonly called the light bulb sea squirt (Reinhardt et al., 2010) due to its zooid appearance marked with white rings around two siphons and pharynx and with white longitudinal lines along the endostyle and dorsal lamina. Zooids usually had white pigmentation around the oral siphon. Zooids were composed of perfectly transparent and colorless tunics, and were usually associated in clusters, forming a colony united by stolonious bases. This species turned out to be an invasive alien species, which was initially reported from Norway (Muller,1776), and thereafter in North East Atlantic (Wirtz, 1998),in South Africa (Robinson et al., 2005) and Connecticut of North West Atlantic (Reinhardt et al., 2010). Thus this species observed from only few ports of Korea was presumed to have been introduced recently from northwest Europe and the Mediterranean in the same way as hull fouling or ballast water. This suspension feeder was usually found attached to disturbed hard substrates between depths of 1-5 m, especially on vertical faces of docks or ropes at the four harbors in Korea.
Korean name: 1*전구멍게(신칭)