검색 전체 메뉴
PDF
맨 위로
OA 학술지
Microbial Removal Using Layered Double Hydroxides and Iron (Hydr)oxides Immobilized on Granular Media
  • 비영리 CC BY-NC
  • 비영리 CC BY-NC
ABSTRACT

The objective of this study was to investigate microbial removal using layered double hydroxides (LDHs) and iron (hydr)oxides (IHs) immobilized onto granular media. Column experiments were performed using calcium alginate beads (CA beads), LDHs entrapped in CA beads (LDH beads), quartz sand (QS), iron hydroxide-coated sand (IHCS) and hematite-coated sand (HCS). Microbial breakthrough curves were obtained by monitoring the effluent, with the percentage of microbial removal and collector efficiency then quantified from these curves. The results showed that the LDH beads were ineffective for the removal of the negatively-charged microbes (27.7%at 1 mM solution), even though the positively-charged LDHs were contained on the beads. The above could be related to the immobilization method, where LDH powders were immobilized inside CA beads with nano-sized pores (about 10 nm); therefore, micro-sized microbes (E. coli = 1.21 ㎛) could not diffuse through the pores to come into contact with the LDHs in the beads, but adhere only to the exterior surface of the beads via polymeric interaction. IHCS was the most effective in the microbial removal (86.0% at 1 mM solution), which could be attributed to the iron hydroxide coated onto the exterior surface of QS had a positive surface charge and, therefore, effectively attracted the negatively-charged microbes via electrostatic interactions. Meanwhile, HCS was far less effective (35.6% at 1 mM solution) than IHCS because the hematite coated onto the external surface of QS is a crystallized iron oxide with a negative surface charge. This study has helped to improve our knowledge on the potential application of functional granular media for microbial removal.


KEYWORD
Microbial removal , Layered double hydroxides , Iron (hydr)oxides , Calcium alginate bead , Iron hydr(oxide)-coated sand
  • 1. Introduction

    Contamination of surface water and groundwater by microbial pathogens is a serious environmental problem, causing deterioration of the quality of drinking water resources and posing a great threat to human health. Inadequate sanitation causes waterborne diseases, leading to a great number of deaths around the world [1]. Understanding of microbial interactions with granular media is important with respect to the removal of microorganisms in water filtration systems. In granular media,the movement of microorganisms is mainly controlled by advective-dispersive transport and attachment to solid matrices [2]. The deposition of microorganisms on granular media is affected by the properties of the granular media (e.g., surface charge and grain size), solution chemistry (e.g., ionic strength, pH and ionic composition) and characteristics of the microorganisms (e.g., cell surface charge and hydrophobicity) [3, 4].

    Point-of-use (POU) water treatment alternatives are of considerable interest as they provide safe drinking water and subsequently prevent water-related diseases. POU treatment could be directly applied to treat the water used at a single tap within a home [5-9]. Layered double hydroxides (LDHs) could be used in POU treatment systems to remove microbial pathogens. LDHs are a class of nanostructured anionic clays, consisting of positively charged brucite-like sheets, which are balanced by the intercalation of anions in the hydrated interlayer regions [10]. Because of their high surface area, large anion exchange capacity,and good thermal stability [11-13], LDHs have been studied for their potential application to microbial removal [14, 15]. Iron (hydr)oxides (IHs) could also be applied to remove microorganisms. Through the modification of the surface charges on granular media with IHs, the microbial removal in water filtration systems could be enhanced [16-18]. Further experiments and quantitative analyses are required to improve our knowledge regarding the microbial removal using these hydroxides.

    The objective of this study was to investigate the microbial removal using LDHs and IHs immobilized onto granular media with three sets of column experiments. The first experiments were performed on calcium alginate beads (CA beads) and LDHs entrapped in CA beads (LDH beads), while the second and third experiments were carried out on iron hydroxide-coated sand (IHCS) and hematite-coated sand (HCS). Microbial break-through curves (BTCs) were obtained by monitoring the effluent, with the percentage of microbial removal and the collector efficiency quantified using the curves.

    2. Materials and Methods

       2.1. Microorganisms

    E. coli ATCC 11105, obtained from the Korea Culture Center for Microorganisms, was used in the experiments. All glassware and materials used in the study were sterilized by autoclaving at 121℃ and 17.6 psi for 20 minutes to prevent any interference from other microorganisms. Initially, the freeze-dried bacteria were revived in 250-mL Erlenmeyer flasks, containing 100 mL of LB medium (tryptone 10 g, yeast extract 5 g, NaCl 5 g in one liter of deionized water at pH of 7.0), for 84 hours at 30℃. Then, one milliliter of culture was transferred to 500 mL of LB broth, and the bacteria incubated for 84 hours at 30℃. The suspension was centrifuged at 4℃ and 10,000 rpm for 15 minutes. The supernatant was removed and replaced with deionized water to prevent growth of the bacteria. The diluted bacteria were re-centrifuged under the same conditions. The centrifuged bacteria were washed three times with deionized water and resuspended in deionized water to an optical density of 0.5 at 600 nm (OD600).

    Transmission electron microscopy (JEM 1010; JEOL, Tokyo, Japan) was used to obtain images of the E. coli cells. The images were imported into an image-processing program (Image-Pro Plus) and analyzed. The average length and diameter of the E.coli were 2.2 and 0.6 ㎛, respectively, which corresponded to an equivalent spherical diameter of 1.21 ㎛. The net surface electrostatic characteristics of the cells were analyzed with an electrophoretic light scattering spectrophotometer (ELS-8000; Otsuka Electronics, Tokyo, Japan). The electrophoretic mobility was determined for the bacterial surface at different NaCl concentrations (1, 10 and 100 mM) and converted to zeta potentials using the Smoluchowski equation. About 10 separate readings were conducted, with the average values presented.

       2.2. Granular Media

    All chemicals used for the experiments were purchased from Sigma Aldrich. LDH powders were prepared by co-precipitating mixtures of magnesium nitrate [Mg(NO3)2·6H2O] and aluminum nitrate [Al(NO3)3·9H2O]. A 700 mL solution (Mg/Al molar ratio = 2) of Mg(NO3)2·6H2O (1 mol) and Al(NO3)3·9H2O (0.5 mol) was added drop wise, using a peristaltic pump (QG400; Fasco, Springfield, MO, USA) at a rate of 3 mL/min, into 1,000 mL of an alkali solution (pH = 13) of sodium hydroxide (NaOH) and sodium carbonate (Na2CO3), with intensive stirring, at room temperature. The resulting precipitates were oven-dried at 65℃ for 18 hours, and then washed thoroughly with deionized water to remove excess sodium, with the final suspensions centrifuged at 8,500 rpm for 20 minutes. The washed precipitates were ovendried again at 65℃ for 24 hours and then pulverized in a ball mill. The Mg-Al LDHs used for the preparation of the LDH beads were finally obtained via calcination at 573 K for 24 hours in an electric muffle furnace (C-FMA; Vision Lab, Seoul, Korea).

    The LDH beads were prepared by entrapping a powdered form of Mg-Al LDHs in a calcium alginate gel. Two grams of sodium alginate powder were added to 200 mL of deionized water at room temperature to prepare a sodium alginate solution. The desired amount of LDHs (8% w/v) was then added to 200 mL alginate solution, with intensive stirring, to obtain a homogeneous suspension. The suspension, contained in a plastic syringe (outlet diameter = 2 mm), was allowed to drop into a stirred reservoir containing 500 mL of 0.3M calcium chloride (CaCl2) solution in order to form 4.15 ± 0.23 mm (n = 90) spherical LDH beads. The beads were allowed to cure in the same CaCl2 solution for 24 hours, with stirring, and then rinsed with deionized water to remove any excess Ca2+.

    Quartz sand (QS) was also used in the experiments (Jumunjin Silica, Kangreung, Korea). Mechanical sieving was conducted with US Standard Sieves Nos. 35 and 10. Sand fractions, with a grain size of 0.5-2.0 mm and a mean diameter of 1.0 mm, were used in the experiments. Before use, the sand was washed twice using deionized water to remove impurities from the surface,with the wet sand autoclaved for 20 minutes at 17.6 psi, cooled to room temperature, and then oven-dried at 105℃ for 1-2 days. For the preparation of the IHCS, FeCl3?6H2O (5.5 g) was dissolved in deionized water (100 mL), with the solution pH adjusted using 6 M NaOH. The QS (200 g) was added to FeCl3?6H2O solution and then mixed in a rotary evaporator (90℃, 80 rpm, 20 minutes) to remove the water in the suspension by heating (Hahnvapor; Hahnshin Scientific Co., Seoul, Korea). The coated sand was dried at 150℃ for 6 hours, washed with deionized water and then dried again under the same conditions. HCS was prepared following the same procedures used for the IHCS. The coated sand was thermally treated at 700℃ for 24 hours, in an electric muffle furnace (C-FMA; Vision Lab), to obtain HCS.

    The powdered forms of the LDHs and IHs (Fig. 1) were characterized using powder X-ray diffractometry (XRD, D5005; Bruker AXS, Berlin, Germany), using Cu Kα radiation. The granular

    media were observed and examined using X-ray microanalysis (Fig. 2). The granular media were mounted on metal stubs using carbon tape and sputter-coated with platinum (approximately 20 nm in thickness). Specimens were examined with a field emission scanning electron microscope (FESEM, Supra 55VP; Carl Zeiss, Oberkochen, Germany), at an accelerating voltage of 3 kV.

       2.3. Column Experiments

    Column experiments were conducted using Plexiglas columns, with an inner diameter of 2.5 cm and a height of 10 cm, packed with the granular media. All the experiments were performed in duplicate (Table 1). The column was connected to a HPLC pump (Series II; Scientific Systems, Inc., Woburn, MA,USA), operating at a rate of 0.3 mL/min. Before the microbial injection, the packed column was flushed upward with 15-20 pore volumes of NaCl solution (1, 10 and 100 mM) to achieve steady state flow conditions. E. coli (0.5 OD600) were introduced downward into the column for 35 minutes, along with NaCl solution.After completing the microbial injection, further NaCl solution was introduced into the column. Effluent samples were collected at regular intervals using an auto collector (Retriever 500; Teledyne Technologies, Inc., Thousand Oaks, CA, USA). Effluents were analyzed for their bacterial concentrations. The microbial concentration was determined by measuring the optical density of the effluent using a UV-visible spectrophotometer (Helios; Thermo Scientific, Waltham, MA, USA) at 600 nm(OD600). The pH and electrical conductivity (EC) of the influent and effluent solutions were measured with a pH probe (9107BN;Orion, Cupertino, CA, USA) and an EC probe (815PDL; Istek, Seoul, Korea), respectively.

       2.4. Data Analysis

    Assuming negligible microbial growth and decay, the onedimensional microbial transport can be described as:

    image

    where C is the microbial concentration in the aqueous phase, D the hydrodynamic dispersion coefficient for microbes, ν the velocity for microbes and λ the attachment (removal) rate coefficient (1/T). The parameters in the transport models were ob

    [Table 1.] Column experimental conditions and the results for Escherichia coli in the granular media

    label

    Column experimental conditions and the results for Escherichia coli in the granular media

    tained by fitting the CXTFIT code [19] to the breakthrough data. The percentage of microbial removal (R) in the effluent was quantified by the following equation:

    image

    where C0 is the initial concentration of microbes and t0 the duration of microbial injection (injection time).

    From colloid filtration theory, the removal of biocolloids/colloids from suspension by attachment to a collector can be described by the collector efficiency (Ce), which denotes the probability that a colloidal particle approaching a collector both collides with and sticks to the collector [20]:

    image

    where η is the collision efficiency, which is the probability that a colloidal particle approaching a collector collides with the collector, and α the sticking efficiency, which is the probability that the particle colliding with the collector sticks to that collector. The collision efficiency is calculated using the following Eq. [21]:

    image

    where AS is the porosity?dependent parameter, NR the aspect ratio, NPe the Peclet number, NvdW the van der Waals number, NA the attraction number and NG the gravity number. The sticking efficiency is determined using the following Eq. [21]:

    image

    where dc is the particle diameter of collector grain, L the column length and n the porosity. The parameters used in the calculation of the collector efficiency are summarized in Table 2.

    [Table 2.] Parameters used in the calculation of the collector efficiency (Ce) for Escherichia coli in the granular media

    label

    Parameters used in the calculation of the collector efficiency (Ce) for Escherichia coli in the granular media

    3. Results and Discussion

       3.1. Microbial BTCs

    The microbial BTCs obtained from the column experiments using the granular media are presented in Fig. 3. With the CA beads and LDH beads (Fig. 3a), the BTCs had well defined single peaks, with different relative peak concentrations depending on the granular medium and ionic strength. The relative peak concentrations ranged from 0.323 to 0.601, with the lowest obtained for 1 mM solution and alginate beads (Ex. 1), and the highest with 10 mM solution and LDH beads (Ex. 4). The transport pa-

    rameters (v and D) obtained from the model fit for the microbial BTCs were 0.167 ± 0.003 cm/min and 0.165 ± 0.074 cm2/min, respectively. The attachment rate coefficients (λ) ranged from 0.0016 to 0.0090 1/min, with the lowest with 10 mM solution and LDH beads (Ex. 4), and the highest for 1 mM solution and CA beads (Ex. 1) (Table 1).

    The microbial BTCs from the experiments with QS and IHCS are given in Fig. 3b. The BTCs also had well-defined single peaks, with the relative peak concentrations ranging from 0.092 to 0.493, with the highest obtained for 1 mM quartz sand (Ex. 5). The values of v and D determined from the BTCs were 0.153 ±0.004 cm/min and 0.061 ± 0.017 cm2/min, respectively. The values of λ was 0.0088 ± 0.0002 and 0.0319 ± 0.0009 1/min with QS and IHCS, respectively, which remained constant, even though the ionic strength of the leaching solution increased. With the HCS (Fig. 3c), the BTCs had relative peak concentrations ranging from 0.335 to 0.437, with the highest for 1 mM solution (Ex. 9) and the lowest for 100 mM solution (Ex. 11). The values of v and D were 0.151 ± 0.001 cm/min and 0.066 ± 0.029 cm2/min, respectively. The values of λ ranged from 0.0056 to 0.0122 1/min, increasing with increasing ionic strength of solution (Table 1).

       3.2. Microbial Removal Percent and Collector Efficiency

    The percentage microbial removal and collector efficiency with 1 mM solution for the granular media are presented in Fig. 4. As shown in Fig. 4a, the average percentage removal (R) was highest for IHCS and lowest for QS. With the CA beads, the average percentage removal was 40.8% (1a = 40.0, 1b = 41.6), but 27.7% with the LDH beads (3a = 26.1, 3b = 29.2). The average percentage removal for QS was 25.2% (5a = 23.5, 5b = 26.9), which was lower than those for CA beads and LDH beads. With the IHCS, the average percentage removal was 86.0% (6a = 85.7, 6b = 86.2), which was about 3.4 times higher than that for QS. The average percentage removal with HCS was 35.6% (9a = 34.1, 9b = 37.0).

    As presented in Fig. 4b, the average collector efficiency (Ce) was lowest with QS and highest with IHCS. With the CA beads, the average collector efficiency was 0.0181 (1a = 0.0177, 1b =0.0186), which was about two times higher than that (= 0.0099) of the LDH beads (3a = 0.0092, 3b = 0.0105). The average collector efficiency for QS was 0.0032 (5a = 0.0030, 5b = 0.0034).With the IHCS, the average collector efficiency was 0.0218 (6a =0.0218, 6b = 0.0217), which was one order of magnitude higher than that of the QS. The average collector efficiency with HCS was 0.0048 (9a = 0.0045, 9b = 0.0050).

    The percentage microbial removal and collector efficiency with the various solutions (1, 10 and 100 mM) are presented in Fig. 5. As shown in Fig. 5a, the average percentage removal with the LDH beads decreased from 27.7 to 3.9% (4a = 3.7, 4b= 4.1) on increasing the ionic strength from 1 to 10 mM. Note:no column result was obtained for LDH beads in 100 mM solution due to unstable behavior of the LDH beads. When NaCl was added to adjust the ionic strength, the Na+ ions partially displace Ca2+ ions from the alginate hydrogel beads [23]. With the IHCS, the average percentage removal remained constant at around 86.0%, even though the ionic strength was increased from 1 to 100 mM. Meanwhile, the average percentage removal with the HCS gradually increased from 35.6 to 53.4% (11a = 54.5, 11b =52.2) with increasing ionic strength from 1 to 100 mM (Table 1).

    As shown in Fig. 5b, the average collector efficiency (Ce) for the LDH beads decreased from 0.0099 to 0.0012 (4a = 0.0011, 4b= 0.0013) with increasing ionic strength from 1 to 10 mM. With the IHCS, the average collector efficiency remained constant at around 0.0222, even though the ionic strength was increased from 1 to 100 mM. The average collector efficiency for HCS increased from 0.0048 to 0.0085 (11a = 0.0087, 11b = 0.0083) with increasing ionic strength from 1 to 100 mM (Table 1).

       3.3. Microbial Removal in Granular Media

    Sodium alginate, a water-soluble salt of alginic acid, is an anionic linear polysaccharide composed of repeated (1-4)-linked D-mannuronic and L-glucuronic acid units. Calcium ions can chelate carboxylate groups, make crosslinks between chains, and form an insoluble hydrogel [24]. CA beads are an anionic biopolymer, with a point of zero charge (pHpzc) of 6.5[25]. The pHpzc is the pH where the net surface charge is equal to zero. The surface charge of CA beads depends on the solution pH. Therefore, CA beads carry a positive charge below the pHpzc, but a negative charge above that. In our experiments with CA beads in 1 mM solution (Ex. 1a and b), where the solution pH was around 7.2, the surface of the CA beads would have been negatively charged, such that the electrostatic interaction between the CA beads and E. coli would be repulsive. Note: E. coli is negatively charged in 1 mM solution (zeta potential = -34.2 mV); that is, the adhesion of E. coli to the surface of CA beads would be unfavorable in terms of their electrostatic interaction. The average percentage removal with CA beads in 1 mM solution was 40.8%(Fig. 4). This removal (adhesion) could be attributed to the polymeric interaction between the polysaccharides of the CA beads and E. coli. Polysaccharides, such as lipopolysaccharides (LPS), and exopolymeric substances (EPS) on bacterial cell surfaces might play major roles in the bacterial adhesion to the surface of CA beads (polysaccharide) via polymeric interaction [26, 27].

    LDHs have pHpzc of between 10 and 11[15]; therefore, they would have been positively charged under our experimental conditions (solution pH = 8.3). The negatively charged E. coli might be effectively attached to the LDHs via electrostatic interaction.In our experiments; however, the LDH beads were not effective for microbial removal (27.7% at 1 mM solution) (Fig.4). This could have been related with how the LDHs were immobilized in the CA beads during the synthesis stage (Fig. 2b). LDH powders were immobilized inside the CA beads, with pore sizes of about 10 nm [28]. Therefore, E. coli (equivalent diameter= 1.21 ㎛) could not diffuse through the pores to come into contact with the LDHs located on the interior of the CA beads, but could only adhere to the exterior surface of the CA beads.

    Of the granular media used in our experiments, IHCS was most effective (86.0% in 1 mM solution) at microbial removal(Fig. 4). This could be attributed to the iron hydroxide (Fe(OH)3) coated on the exterior surface of QS having a positive surface charge (zeta potential = 9.7 mV), which effectively attracted the negatively-charged microbes. Note: the iron hydroxide was poorly crystallized, as indicated by the XRD data (Fig. 1b). Meanwhile, HCS was far less effective (35.6% in 1 mM solution) at microbial removal than IHCS (Fig. 4). This could be ascribed to the hematite (Fe2O3) coated on the external surface of QS being a crystallized iron oxide (Fig. 1c) with a negative surface charge(zeta potential = -1.8 mV).

    The average percentage removals in 1 mM solution for both CA beads and LDH beads were higher than those in 10 mM solution (Fig. 5). This could be ascribed to the bacterial polysaccharides being fully extended from the cell surface at low ionic strengths, resulting in increased bacterial adhesion to the surfaces[29]. With the IHCS, the percentage removal remained constant on increasing the ionic strength from 1 to 100 mM (Fig.5). This might be attributable to E. coli having many carboxyl (COOH) groups on its outer membrane [30] and; thus, could be adsorbed to IHCS via an inner-sphere complex, with minimal impact due to the ionic strength [31]. With the HCS, the percentage removal increased with increasing ionic strength (Fig.5). This could be explained by the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory [32], because both HCS and bacteria are negatively-charged. An increase in the ionic strength could lead to a decrease in the thickness of the electrical double layers and in the average distance between the HCS and bacteria, subsequently resulting in the promotion of bacterial removal with HCS

    4. Conclusions

    The microbial removals using LDHs and IHs immobilized on granular media were investigated using column experiments.The results showed that LDH beads were not effective for the removal of negatively-charged microbes, even though the positively-charged LDHs were contained in the beads. This could be related to the immobilization method, where LDH powders were immobilized inside the CA beads, with nano-sized pores; therefore, micro-sized microbes could not diffuse through the pores to come into contact with the LDHs in the bead, but would only adhere to the exterior surface of the bead via polymeric interaction. IHCS was most effective for the microbial removal, which could be attributed to the iron hydroxide coated on the exterior surface of the QS having a positive surface charge; therefore, would be effectively attracted to the negatively-charged microbes via electrostatic interaction. Meanwhile, HCS was far less effective than IHCS because the hematite coated on the external surface of QS is a crystallized iron oxide with a negative surface charge. This study has helped improve our knowledge on the potential application of functional granular media for microbial removal.

참고문헌
  • 1. Souter PF, Cruickshank GD, Tankerville MZ 2003 Evaluation of a new water treatment for point-of-use household applications to remove microorganisms and arsenic from drinking water. [J. Water Health] Vol.1 P.73-84 google
  • 2. Hornberger GM, Mills AL, Herman JS 1992 Bacterial transport in porous media: Evaluation of a model using laboratory observations. [Water Resour. Res.] Vol.28 P.915-923 google cross ref
  • 3. Gannon JT, Manilal VB, Alexander M 1991 Relationship between cell surface properties and transport of bacteria through soil. [Appl. Environ. Microbiol.] Vol.57 P.190-193 google
  • 4. Fontes DE, Mills AL, Hornberger GM, Herman JS 1991 Physical and chemical factors influencing transport of microorganisms through porous media. [Appl. Environ. Microbiol.] Vol.57 P.2473-2481 google
  • 5. Lantagne DS, Blount BC, Cardinali F, Quick R 2008 Disinfection by-product formation and mitigation strategies in point-ofuse chlorination of turbid and non-turbid waters in Western Kenya. [J. Water Health] Vol.6 P.67-82 google cross ref
  • 6. Brownell SA, Chakrabarti AR, Kaser FM 2008 Assessment of a low-cost point-of-use ultraviolet water disinfection technology. [J. Water Health] Vol.6 P.53-65 google cross ref
  • 7. Doocy S, Burnham G 2006 Point-of-use water treatment and diarrhoea reduction in the emergency context: An effectiveness trial in Liberia. [Trop. Med. Int. Health] Vol.11 P.1542-1552 google cross ref
  • 8. Gurian PL, Small MJ 2002 Point-of-use treatment and the revised arsenic MCL. [J. Am. Water Works Assoc.] Vol.94 P.101-108 google
  • 9. Slotnick MJ, Meliker JR, Nriagu JO 2006 Effects of time and pointof-use devices on arsenic levels in Southeastern Michigan drinking water USA. [Sci. Total Environ.] Vol.369 P.42-50 google cross ref
  • 10. Goh KH, Lim TT, Dong Z 2008 Application of layered double hydroxides for removal of oxyanions: a review. [Water Res.] Vol.42 P.1343-1368 google cross ref
  • 11. Cavani F, Trifiro F, Vaccari A 1991 Hydrotalcite-type anionic clays: Preparation properties and applications. [Catal. Today] Vol.11 P.173-301 google cross ref
  • 12. Vaccari A 1998 Preparation and catalytic properties of cationic and anionic clays. [Catal. Today] Vol.41 P.53-71 google cross ref
  • 13. Das NN, Konar J, Mohanta MK, Srivastava SC 2004 Adsorption of Cr(VI) and Se(IV) from their aqueous solutions onto Zr4+-substituted ZnAl/MgAl-layered double hydroxides: Effect of Zr4+ substitution in the layer. [J. Colloid Interface Sci.] Vol.270 P.1-8 google cross ref
  • 14. Jin S, Fallgren PH, Morris JM, Chen Q 2007 Removal of bacteria and viruses from waters using layered double hydroxide nanocomposites. [Sci. Tech. Adv. Mater.] Vol.8 P.67-70 google cross ref
  • 15. You Y, Vance GF, Sparks DL, Zhuang J, Jin Y 2003 Sorption of MS2 bacteriophage to layered double hydroxides: effects of reaction time pH and competing anions. [J. Environ. Qual.] Vol.32 P.2046-2053 google cross ref
  • 16. Foppen JW, Liem Y, Schijven J 2008 Effect of humic acid on the attachment of Escherichia coli in columns of goethite-coated sand. [Water Res.] Vol.42 P.211-219 google cross ref
  • 17. Lukasik J, Cheng YF, Lu F, Tamplin M, Farrah SR 1999 Removal of microorganisms from water by columns containing sand coated with ferric and aluminum hydroxides. [Water Res.] Vol.33 P.769-777 google cross ref
  • 18. Kim SB, Park SJ, Lee CG, Choi NC, Kim DJ 2008 Bacteria transport through goethite-coated sand: Effects of solution pH and coated sand content. [Colloids Surf. B. Biointerfaces] Vol.63 P.236-242 google cross ref
  • 19. Toride N, Leij FJ, Van Genuchten MT 1995 The CXTFIT code for estimating transport parameters from laboratory or field tracer experiments. google
  • 20. Cail TL, Hochella Jr MF 2005 The effects of solution chemistry on the sticking efficiencies of viable Enterococcus faecalis: An atomic force microscopy and modeling study. [Geochim. Cosmochim. Acta] Vol.69 P.2959-2969 google cross ref
  • 21. Tufenkji N, Elimelech M 2004 Correlation equation for predicting single-collector efficiency in physicochemical filtration in saturated porous media. [Environ. Sci. Technol.] Vol.38 P.529-536 google cross ref
  • 22. Martinez-Salas E, Martin JA, Vicente M 1981 Relationship of Escherichia coli density to growth rate and cell age. [J. Bacteriol.] Vol.147 P.97-100 google
  • 23. Velings NM, Mestdagh MM 1995 Physico-chemical properties of alginate gel beads. [Polym. Gels Networks] Vol.3 P.311-330 google cross ref
  • 24. Banerjee A, Nayak D, Lahiri S 2007 A new method of synthesis of iron doped calcium alginate beads and determination of iron content by radiometric method. [Biochem. Eng. J.] Vol.33 P.260-262 google cross ref
  • 25. Escudero C, Fiol N, Villaescusa I, Bollinger JC 2009 Arsenic removal by a waste metal (hydr)oxide entrapped into calcium alginate beads. [J. Hazard. Mater.] Vol.164 P.533-541 google cross ref
  • 26. Tsuneda S, Aikawa H, Hayashi H, Yuasa A, Hirata A 2003 Extracellular polymeric substances responsible for bacterial adhesion onto solid surface. [FEMS Microbiol. Lett.] Vol.223 P.287-292 google cross ref
  • 27. Hori K, Matsumoto S 2010 Bacterial adhesion: From mechanism to control. [Biochem. Eng. J.] Vol.48 P.424-434 google cross ref
  • 28. Klein J, Stock J, Vorlop KD 1983 Pore size and properties of spherical Ca-alginate biocatalysts. [Eur. J. Appl. Microbiol. Biotechnol.] Vol.18 P.86-91 google cross ref
  • 29. Abu-Lail NI, Camesano TA 2002 Elasticity of pseudomonas putida KT2442 surface polymers probed with single-molecule force microscopy. [Langmuir] Vol.18 P.4071-4081 google cross ref
  • 30. Katowsky M, Sabisch A, Gutberlet T, Bradaczek H 1991 Molecular modelling of bacterial deep rough mutant lipopolysaccharide of Escherichia coli. [Eur. J. Biochem.] Vol.197 P.707-716 google cross ref
  • 31. Cornell RM, Schwertmann U 1996 The iron oxides: structureproperties reactions occurrence and uses. google
  • 32. Poortinga AT, Bos R, Norde W, Busscher HJ 2002 Electric double layer interactions in bacterial adhesion to surfaces. [Surf. Sci. Rep.] Vol.47 P.1-32 google cross ref
이미지 / 테이블
  • [ Fig. 1. ]  X-ray diffraction patterns for the powdered forms of: (a) layered double hydroxides (b) iron hydroxides and (c) hematite.
    X-ray diffraction patterns for the powdered forms of: (a) layered double hydroxides (b) iron hydroxides and (c) hematite.
  • [ Fig. 2. ]  Field emission scanning electron microscope images: (a) cross-sectional view of a calcium alginate bead (bar = 20 ㎛) (b) crosssectional view of layered double hydroxides immobilized on a calcium alginate bead (bar = 2 ㎛) (c) surface view of iron hydroxide-coated sand (bar = 100 ㎛) and (d) surface view of hematite-coated sand (bar = 200 nm).
    Field emission scanning electron microscope images: (a) cross-sectional view of a calcium alginate bead (bar = 20 ㎛) (b) crosssectional view of layered double hydroxides immobilized on a calcium alginate bead (bar = 2 ㎛) (c) surface view of iron hydroxide-coated sand (bar = 100 ㎛) and (d) surface view of hematite-coated sand (bar = 200 nm).
  • [ Table 1. ]  Column experimental conditions and the results for Escherichia coli in the granular media
    Column experimental conditions and the results for Escherichia coli in the granular media
  • [ Table 2. ]  Parameters used in the calculation of the collector efficiency (Ce) for Escherichia coli in the granular media
    Parameters used in the calculation of the collector efficiency (Ce) for Escherichia coli in the granular media
  • [ Fig. 3. ]  Breakthrough curves of Escherichia coli obtained from column experiments: (a) calcium alginate beads and layered double hydroxides immobilized on calcium alginate beads (Ex. 1a-4b) (b) quartz sand and iron hydroxide-coated sand (Ex. 5a-8b) and (c) hematite-coated sand (Ex. 9a-11b).
    Breakthrough curves of Escherichia coli obtained from column experiments: (a) calcium alginate beads and layered double hydroxides immobilized on calcium alginate beads (Ex. 1a-4b) (b) quartz sand and iron hydroxide-coated sand (Ex. 5a-8b) and (c) hematite-coated sand (Ex. 9a-11b).
  • [ Fig. 4. ]  Percentage microbial removal (a) and collector efficiency (b) of the granular media (1 mM solution).
    Percentage microbial removal (a) and collector efficiency (b) of the granular media (1 mM solution).
  • [ Fig. 5. ]  Percentage microbial removal (a) and collector efficiency (b) of the granular media under different ionic strengths.
    Percentage microbial removal (a) and collector efficiency (b) of the granular media under different ionic strengths.
(우)06579 서울시 서초구 반포대로 201(반포동)
Tel. 02-537-6389 | Fax. 02-590-0571 | 문의 : oak2014@korea.kr
Copyright(c) National Library of Korea. All rights reserved.