대황(Eisenia bicyclis) 추출액의 항산화 및 항염증 활성에 대한 유산균 발효의 영향

Effects of Lactic Acid Bacterial Fermentation on the Antioxidant and Anti-inflammatory Activity of Brown Algae Eisenia bicyclis Extract

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  • ABSTRACT

    This study was conducted to evaluate the effect of lactic acid bacterial fermentation on the anti-oxidant and antiinflammatory activity of an edible brown alga, Eisenia bicyclis. Lactic acid bacteria were inoculated into and cultivated in E. bicyclis water extract. The antioxidant activity of the extract was assayed before and following fermentation. Antioxidant activity was determined by assaying the levels of radical scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and alkyl radical. The lactic acid bacterial fermentation of E. bicyclis extract resulted in enhanced antioxidant activity. The greatest enhancement of antioxidant activity was seen in the DPPH radical scavenging assay, in which E. bicyclis extract was fermented by Pediococcus pentosaceus MBP-34 strain for 12 h. This fermented extract also exhibited higher inhibitory activity (96.66%) on nitric oxide production compared with other lactic acid bacterial fermented extracts or raw extract (189.60%). In conclusion, fermentation by bacterial strain is an attractive strategy for developing value-added food ingredients.


  • KEYWORD

    Anti-inflammatory activity , Antioxidant activity , Eisenia bicyclis , Fermentation , Lactic acid bacteria

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  • [Table 1.] Change of total phenolic contents in Eisenia bicyclis water extract by lactic acid bacteria fermentation
    Change of total phenolic contents in Eisenia bicyclis water extract by lactic acid bacteria fermentation
  • [Fig. 1.] Effect of lactic acid bacteria fermentation on DPPH radical scavenging activity of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. □, ascorbic acid (500 μg/mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/ mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). L.05, Lactobacillus brevis MBP-05; L.06, L. plantarum MBP-06; P.10, Pediococcus pentosaceus MBP- 10; L.17, L. brevis MBP-17; L.20, L. brevis MBP-20; L.21, L. plantarum MBP-21; P.34, P. pentosaceus MBP-34; L.01, L. plantarum AML-01; L.15, L. plantarum AML-15; L.02, L. fermentum MBP-02.
    Effect of lactic acid bacteria fermentation on DPPH radical scavenging activity of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. □, ascorbic acid (500 μg/mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/ mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). L.05, Lactobacillus brevis MBP-05; L.06, L. plantarum MBP-06; P.10, Pediococcus pentosaceus MBP- 10; L.17, L. brevis MBP-17; L.20, L. brevis MBP-20; L.21, L. plantarum MBP-21; P.34, P. pentosaceus MBP-34; L.01, L. plantarum AML-01; L.15, L. plantarum AML-15; L.02, L. fermentum MBP-02.
  • [Fig. 2.] Effect of lactic acid bacteria fermentation on hydroxyl radical scavenging activity of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. □, ascorbic acid (500 μg/mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/ mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). Bacterial strains were the same as listed in Fig. 1.
    Effect of lactic acid bacteria fermentation on hydroxyl radical scavenging activity of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. □, ascorbic acid (500 μg/mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/ mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). Bacterial strains were the same as listed in Fig. 1.
  • [Fig. 3.] Effect of lactic acid bacteria fermentation on alkyl radical scavenging activity of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. □, ascorbic acid (500 μg/mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/ mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). Bacterial strains were the same as listed in Fig. 1.
    Effect of lactic acid bacteria fermentation on alkyl radical scavenging activity of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. □, ascorbic acid (500 μg/mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/ mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). Bacterial strains were the same as listed in Fig. 1.
  • [Fig. 4.] Effect of lactic acid bacteria fermentation on cell cytotoxicity (Raw 264.7 cells) of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. □, ascorbic acid (500 μg/mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/ mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). Bacterial strains were the same as listed in Fig. 1.
    Effect of lactic acid bacteria fermentation on cell cytotoxicity (Raw 264.7 cells) of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. □, ascorbic acid (500 μg/mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/ mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). Bacterial strains were the same as listed in Fig. 1.
  • [Fig. 5.] Effect of lactic acid bacteria fermentation on cell (Raw 264.7 cells) based nitric oxide assay of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. ■, blank; ■, lipopolysaccharide(LPS, 1 μg/mL); □, A.C (ascorbic acid, 500 μg/ mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). Bacterial strains were the same as listed in Fig. 1.
    Effect of lactic acid bacteria fermentation on cell (Raw 264.7 cells) based nitric oxide assay of Eisenia bicyclis water extract. Results are means ±SD of triplicate data. ■, blank; ■, lipopolysaccharide(LPS, 1 μg/mL); □, A.C (ascorbic acid, 500 μg/ mL); ■, EWE (E. bicyclis water extract, 500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 12 h (500 μg/mL); ■, E. bicyclis water extract fermented by lactic acid bacterial strains for 24 h (500 μg/mL). Bacterial strains were the same as listed in Fig. 1.