Effects of Galgunhaejutang on Alcohol Consumption in C57BL/6 Mice

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  • ABSTRACT

    Objectives :

    Galgunhaejutang extract (GHT) is a Traditional Korean medical herbal mixture that has been tradtionally used to treat alcohol consumption. This study was preformed to evaluate the effects of GHT on alcohol consumption in C57BL/6 mice.

    Methods :

    Sixty three C57BL/6 mice were alcohol dependence-induced by limited access paradigm. Water, GHT 0.688 g/kg (GHT-L), or GHT 3.45 g/kg (GHT-H) were administrated for 10 days. The amounts of alcohol consumption for 2 hours, water consumption for 22 hours, food intake for 24 hours and body weight were measured.

    Results :

    There weren't significant differences in 2 hours of alcohol consumption, 22 hours of water consumption,24 hours of food intake and body weight for ten days between vehicle group and GHT-L or GHT-H group.

    Conclusions :

    Further studies employing multi-dose and long term administration of GHT (more than 10 days) might be of benefit.


  • KEYWORD

    Korean traditional medicine , Herbal mixture , Alcohol consumption , C57BL/6.

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  • [Table 1.] Contents of Galgunhaejutang
    Contents of Galgunhaejutang
  • [Table 2.] Summary of Experimental Phase from Day 1 to Day 47
    Summary of Experimental Phase from Day 1 to Day 47
  • [Fig. 1.] Effect of GHT-L, GHT-H on alcohol consumption in C57BL/6 mice during 2-hr access to alcohol after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Significant change was observed between vehicle D4 and high dose group D4 (p=0.043) with repeated measure ANOVA and independent t-test with vehicle group, *p<0.05. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.
    Effect of GHT-L, GHT-H on alcohol consumption in C57BL/6 mice during 2-hr access to alcohol after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Significant change was observed between vehicle D4 and high dose group D4 (p=0.043) with repeated measure ANOVA and independent t-test with vehicle group, *p<0.05. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.
  • [Fig. 2.] Effect of GHT-L, GHT-H on water intake in C57BL/6 mice during 22-hr access to water after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Non-significant with repeated measure ANOVA. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.
    Effect of GHT-L, GHT-H on water intake in C57BL/6 mice during 22-hr access to water after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Non-significant with repeated measure ANOVA. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.
  • [Fig. 3.] Effect of GHT-L, GHT-H on alcohol preference (alcohol consumption/water+alcohol consumption) in C57BL/6 mice during limited access to alcohol and water after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Non-significant with repeated measure ANOVA. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.
    Effect of GHT-L, GHT-H on alcohol preference (alcohol consumption/water+alcohol consumption) in C57BL/6 mice during limited access to alcohol and water after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Non-significant with repeated measure ANOVA. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.
  • [Fig. 4.] Effect of GHT-L, GHT-H on food intake in C57BL/6 mice during 24-hr access to food after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Non-significant with repeated measure ANOVA. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.
    Effect of GHT-L, GHT-H on food intake in C57BL/6 mice during 24-hr access to food after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Non-significant with repeated measure ANOVA. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.
  • [Fig. 5.] Effect of GHT-L, GHT-H on body weight in C57BL/6 mice after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Non-significant with repeated measure ANOVA. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.
    Effect of GHT-L, GHT-H on body weight in C57BL/6 mice after GHT administration (g/kg). Valuse are mean±SD for 21 mice. Non-significant with repeated measure ANOVA. Base: mean of two days just before starting drug administration, D2, D4, D6, D8, D10: mean of two days when drug was administered.