Boil-off Loss Ratio of Cocoon Shell as a Selection Criterion in the Newly Developed Bivoltine Silkworm (
Bombyx mori L.) Hybrids
- Author: Seetharamulu J., Seshagiri S.V., Raju P.J.
- Organization: Seetharamulu J.; Seshagiri S.V.; Raju P.J.
- Publish: International Journal of Industrial Entomology Volume 26, Issue1, p13~21, 31 March 2013
Boil-off loss ratio is one of the most important economic traits to be considered during the course of silkworm breeding. The boil-off loss ratio varies among the breeds and seasons. The present study was undertaken with 5 oval and 5 peanut breeds and twenty five hybrids involving these parents to select the promising hybrids with desired boil-off loss ratio. Accordingly, studies were undertaken to estimate the heterosis and heterobeltiosis. It was observed that majority of the hybrids revealed negative heterosis for boil-off loss ratio which is desirable. Based on the heterosis and heterobeltiosis, among all the hybrids, the hybrid APBRO5 x APBRD5 exhibited highest negative heterosis for boil-off loss ratio and also exhibited highest EI value (64.5) for the quantitative and qualitative traits. Based on the hybrids performance, boil？off loss ratio, heterosis and heterobeltiosis and evaluation index, the hybrids viz. APBRO5 x APBRD5, APBRO1 x APBRD1 and APBRO3 x APBRD4 were identified for commercial exploitation.
Boil-off loss ratio , Bivoltine silkworm hybrids , Evaluation index , Heterosis , Heterobeltiosis
To face the global competitiveness in silk production there is a need to improve the quality of the raw silk and as such the sericulture industry requires good quality of silk to produce internationally gradable silk fabric to meet the requirement of the consumers across the world resulting in increased foreign exchange. To meet the specific requirement of the silk industry, the silkworm breeders have developed various hybrids such as productive breeds, robust breeds, breeds with special traits and sex limited races etc. Keeping this in view, most of the silkworm breeders have been concentrating on boil-off loss ratio to produce good quality silk as the boil-off loss ratio is one of the most important qualitative trait in silkworm breeding. The silkworm
Bombyx mori L.spins a shell by extruding silk bave at the end of its larval period. The silk bave is composed of two silk proteins namely fibroin and sericin and also contains a small quantity of fatty, waxy, colouring and mineral matters. The fibroin protein occupies the middle portion of the silk bave representing 70-80 % of its weight and surrounded by three layers of sericin which is representing 20-30 % of the weight. The fatty, waxy, colouring and mineral matters forms a very small part of the silk bave which is not more than 2-3 %.
The main silk substance fibroin is insoluble in alkaline hot water, whereas the sericin (silk gum) is easily soluble in boiling alkaline soap solution (Sadov
et al., 1978). Degumming is the process of removal of sericin in boiling soap solution. The cocoon shell has more boil-off loss percentage when compared to the raw silk. The percentage of boil-off loss ratio has paramount importance in reeling and weaving activities (Kannan, 1986).
The loss of sericin varies in different races and also there is difference in the effect of sericin loss from raw silk on weaving and other post weaving process due to degumming (Sinha
et al., 1992). The boil-off loss ratio for bivoltine is found to be 24 % and it is higher in polyvoltines (Sidhu and Sonwalkar, 1969). The boil-off loss ratio varies according to seasons is influenced by the environment (Sonwalkar, 1969). It is essential to study the boil-off loss ratio with reference to cocoon shell, as it is the basic raw material for the raw silk. The present study was aimed in estimating the boil-off loss ratio in newly developed hybrids as well as their parents for selecting the promising bivoltine hybrids for the benefit of the silk industry.
Five oval breeds
viz.APBRO1, APBR02, APBR03, APBRO4 and APBRO5 (APBRO means Andhra Pradesh Boil ？ off loss Ratio Oval breeds) and five dumbbell breeds viz.APBRD1, APBRD2, APBRD3, APBRD4 and APBRD5 (APBRD means Andhra Pradesh Boil ？ off loss Ratio Dumbbell breeds) were selected for the study. All these parental breeds (5 oval and 5 dumb-bell) and twenty five hybrids were reared by the standard rearing techniques (Basavaraja and Dandin, 2002). After cocoon formation the sexseparation and assessment, 60 cocoon shells (30 each of female and male) were selected from each breed/ hybrid separately. Three replications were maintained each having 10 males and 10 females for conducting the experiment. Degumming was carried out by boiling the cocoon shells in soap solution using a standard procedure (Basavaraja et al., 2000). Two step boiling method as explained below was employed for the degumming of the cocoon shells and used neutral liquid soap for boiling. A quantity of 7g soap was dissolved in 1 liter of water for degumming and the material: liquor ratio was maintained as 1:40.
The liquor solution of the soap and soda was boiled in a copper vessel. When the boiling media solution reached the boiling point (>90℃), the cocoon shell sample bags were immersed completely in boiling media kept tightly closed for 40 minutes. At an interval of 10 minutes, the sample bags were turned up and down for uniform and effective degumming.
In another container, the boiling media of the required concentration was prepared and kept ready at boiling point (>90℃), to transfer the sample material after the first boiling. Immediately after 40 minutes of the first boiling, the sample bags were removed and squeezed to remove the excess liquor solution and transferred to the second bath. The degumming was carried out for another 40 minutes similar to the first degumming as explained above.
Immediately after the second boiling, the sample bags with the degummed shells were rinsed for a minute in an already prepared boiling alkaline solution of 0.1 % sodium carbonate.
After rinsing in 0.1 % sodium carbonate solution, the sample bags with cocoon shells were washed thoroughly (for 15 minutes) in running water using a washing machine. Then, they were dried in the washing machine itself for 5 minutes to remove the excess moisture.
The degummed cocoon shells were transferred to perforated paper envelops and kept for drying in the oven at 105℃ for 5 hours. The dried sample packets were transferred to the desiccator for half an hour for absorption of excess moisture before recording the dry weight of the degummed silk.
The boil-off loss ratio percentage was calculated by using the formula:
The heterosis and heterobeltiosis were calculated by using the following formulae:
Heterobeltiosis (over-dominance) over better parental value
Multiple Trait Evaluation Index (Mano
et al., 1992) was calculated by using the following formula:
A = Value obtained for a particular trait of a particular hybrid combination
B = Mean value of the particular trait of all the
C = Standard deviation
10 = Standard unit and
50 = Fixed value
The boil-off loss ratio for the oval lines ranges from 24.02 % to 25.54 %. The variation in boil-off loss ratio among oval lines was less. Among oval lines the low boil-off loss ratio was recorded in APBRO1 (24.02 %) and maximum in APBRO2 (25.54 %). The average boil-off loss ratio of oval lines was 24.99% (Table 1). However, the entire oval breeds shown
slight variation among them.
Among dumbbell lines the low boil-off loss ratio was recorded in APBRD3 (24.82%) followed by and maximum boil-off loss ratio was recorded in APBRD5 (25.22%). The average boil-off loss ratio of dumbbell lines was 25.00%.
In case of hybrids the minimum boil-off loss ratio was recorded in APBRO1 × APBRD1 (23.34%) and maximum was recorded in APBRO2 × APBRD2 (25.75%) as against 24.32% in the control hybrid APS5 × APS4 (Table 2). Among the hybrid combinations, with regard to heterosis over MPV maximum negative heterosis was recorded in APBRO1 x APBRD1 (-5.18) followed by APBRO3 x APBRD4 (-4.89), APBRO5 x APBRD1 (-4.66) and APBRO3 x APBRD5 (-4.12) where as minimum was in APBRO2 x APBRD5 (-0.08). With regard to heterosis over BPV, maximum negative heterosis was recorded in APBRO5 x APBRD1 (-4.57) followed by APBRO3 x APBRD4 (-4.35), APBRO3 x APBRD5 (-4.03), APBRO4 x APBRD5 (-3.18) and APBRO5 x APBRD5 (-2.93) where as minimum was in APBRO5 x APBRD2 (-0.52). However, eight hybrid combinations have recorded positive boil-off loss ratio over MPV and ten hybrid combinations over BPV which is not desirable for the industry (Table 3). All the hybrid combinations that are involved with the female component of APBRO3, APBRO5 and APBRO4 are showing negative heterosis and heterobeltiosis for the boil-off loss ratio. The performance of these lines regarding boil-off loss ratio is more as pure lines and as a hybrid it is less when compared to the other hybrid combinations which clearly indicates that, these are best combiners regarding the negative heterosis towards this trait. The superiority of the hybrids over parental strains is undoubtedly due to variable magnitude of heterosis for the quantitative and qualitative characters in silkworm and the results of present study are corroborating the findings of Osawa and Harada (1944), Katsumata (1948) and Yokoyama (1962). It has been pointed out by Toyama (1906) that the F1 hybrids in silkworm,
Bombyx moriin several aspects are superior to their pure line parents and
the present results are in support with the findings of Yokoyama (1979) and Singh & Hirobe (1964).
Low boil-off loss ratio improves reeling qualities and it is manifested by dominant genes, while recessive genes act in the opposite direction (Gamo and Hirabayashi, 1984). In sericulturally advanced
countries, the silkworm breeders have developed productive breeds / hybrids with low boil of ratio and quality silk (Gamo and Hirabayashi, 1983; Kurasawa, 1968). During the course of breeding, the boil off loss ratio with reference to cocoon shell has been given utmost importance along with other quantitative and qualitative traits (Gamo and Ichiba (1971); Harada, 1961; Mano
et al., 1988; Yokoyama, 1959). The analysed data on the performance of the parents and hybrids in the expression of boil-off loss ratio corroborate with earlier findings of Sidhu and Sonwalker (1969), Sinha et al., 1992 and Sonwalker (1969). In the present study, less variation was observed among the oval (24.99%) and dumbbell (25.0%) lines regarding the boil- off loss ratio, whereas in hybrids it is 24.11%.
The phenomenon of heterosis and overdominance in conjunction with the expression of boil-off loss ratio analysed in the hybrids under present study facilitated procedures to identify the promising hybrids. Further, the more uniformity in the expression of this trait in hybrids than the parents is one of the desirable features to understand the genetic constitution of the hybrids for their commercial exploitation as evidenced by the mean values computed for boil-off loss ratio. For this trait, negative heterosis is desirable. For instance, high magnitude of negative heterosis was recorded in the combinations of APBRO5 x APBRD5, APBRO1 x APBRD1 and APBRO3 x APBRD4 which could be attributed to the higher mid parental values. The heterosis expressed is variable in different hybrids and these results are in confirmation with the findings of Gamo and Hirabayashi (1983). In certain hybrids, remarkably less heterosis was noticed and very often the hybrids were intermediate between parents for this trait. The result of the present study indicated the manifestation of heterosis in different hybrid combinations for this trait. Positive correlation that existed between boil- off loss ratio and cocoon shell weight of parental breeds clearly confirms the earlier findings of Gamo and Hirabayashi (1983). Improvement of boil-off loss ratio towards low value can be achieved through selection by choosing the crossing types showing higher negative heterosis for this trait. Based on the hybrids performance, boil-off loss ratio, heterosis and heterobeltiosis and evaluation index, the hybrids APBRO5 x APBRD5 followed by APBRO1 x APBRD1, APBRO3 x APBRD4 were identified for further commercial exploitation.
[Table 1.] Mean performance of ten parental breeds
[Table 2.] Mean Boil-off loss ratio of twenty five hybrids
[Table 3.] Heterosis and Over-dominance for Boil-off loss ratio in hybrids
[Table 4.] Performance of the silkworm hybrids
[Table 5.] Evaluation Index values of silkworm hybrids for each of the trait