Organic Acid Extraction of Fluoride from Antarctic Krill Euphausia superba

  • cc icon
  • ABSTRACT

    The amount of fluoride removed from Antarctic krill via organic acid extraction depends on the extraction time, concentration, extraction volume, and the nature of the acid itself. The fluoride content in Euphausia superba was determined by measuring the concentration of fluoride ion in acidic extracts using an ion-selective electrode. The best results were obtained by adding 50 mL of 0.01 M citric acid to 0.3-0.5 g of krill and extracting for 5 min. Under these conditions, recoveries of fluoride from frozen whole krill and krill meat were 95.6-99.5% and 97.5-101.3%, respectively. The dry basis fluoride contents of krill by-product, krill meat and the boiled krill were 705, 298, and 575 ppm, respectively. These levels were significantly reduced by citric acid extraction.


  • KEYWORD

    Fluoride Reduction , Antarctic Krill , Organic acids , Citric acid

  • Introduction

    The Antarctic krill Euphausia superba has been the most abundant species constituting marine biomass since the 1970s and occurs in the greatest numbers in the Antarctic Ocean. Its biomass as a single species has been estimated at approximately 60-150 million tons (Nicol and Foster, 2003). Given these plentiful resources, krill has been recognized as a potential food resource and alternative protein source for humans. Most of the commercially caught Antarctic krill are immediately processed into frozen blocks onboard the fishing vessel to be consumed primarily as recreational fishing bait. Limited amounts of krill meat are also used as fish food in aquaculture (Nicol et al., 2000).

    Despite its huge biomass, Antarctic krill has not been a traditional food in the human diet because of its high fluorine content. While small amounts of fluoride in food have been shown to help prevent tooth decay, too much fluoride in the diet or long-term excessive intake of fluoride can result in macular teeth (Arthur, 1971).

    Both Meganyctiphanes norvegica and E. superba contain high amounts of fluoride. M. norvegica contains 1,300-2,400 mg/kg dry matter (Soevik and Braekkan, 1979), while E. superba contains approximately 1,137 mg F/kg dry weight (Malde et al., 2001). Kumagai (1984) measured the fluoride content of E. superba and tiger prawn Penaeus japonica at 490 and 1.6 μg/g, respectively, based on wet weight. Fluorine is a component of the krill exoskeleton and the natural amount of fluorine in whole-body krill has been reported as 1,009-7,806 ppm. In krill meat (KM), the amount of fluorine ranges from 4.5 to 570 ppm dry weight (Zhang et al., 1993). The exoskeleton and cephalothorax can contain approximately 200 ppm dry weight (Szewielow, 1981). The relatively high fluoride content of krill makes it questionably suitable as a large-scale protein source in human food and animal feed. However, Antarctic krill have attracted some attention as a potential, indirect protein resource for humans (Soevik and Braekkan, 1979).

    Several reports describing the removal and/or quantitation of fluoride in krill and other foods have been published. A simple, rapid, and reliable analytical procedure for determining fluoride levels in biological samples employs alkali fusion and fluoride ion-selective electrodes (Kumagai, 1984; Malde et al., 2001). Kim et al. (1990) studied the migration of fluoride from chitinous components into the muscle of frozen krill (FK) during thawing and reviewed an electrocondensation method as a means of reducing the fluoride content of Antarctic krill. Fluorine removal during the production of krill paste and krill protein concentrates was studied by Filho (1993). The amount of soluble protein in krill muscle depends on pH, ion species, and the ionic strength of the extracting solutions (Park et al., 1988).

    This report details the removal of fluoride from Antarctic krill using an optimized organic acid extraction. Such a pretreatment would allow krill to be used as a protein substitute in human food and aquacultural fish meal.

    Materials and Methods

      >  Sample preparation

    Antarctic krill Euphausia superba were caught in March 2010 by Dong Won Fisheries Co., Ltd. FK, KM, krill by-product (KB), and boiled krill (BK) were produced onboard the fishing vessel immediately after the catch and were frozen to -35℃. Samples were lyophilized or dried and stored at -35℃ until analysis.

      >  Measuring fluoride levels by alkali fusion

    The fluoride content of the krill was determined using a method developed by Malde et al. (2001). Approximately 0.25-0.50 g samples were weighed directly into nickel crucibles. The samples were covered with 2.5-5.0 mL of 8 M sodium hydroxide, depending on the amount of sample. The crucibles were put on a hot plate and evaporated to dryness before being put into a muffle furnace for combustion. The muffle furnace temperature was set at 200℃ for approximately 16 h and then raised to 525℃ and held for another 3 h. The crucibles were cooled, 10-15 mL of distilled water was added, and the crucibles were placed on a hotplate to aid the dissolution of the fusion cake. After approximately 2 h, the sample solutions were transferred to 50 mL plastic tubes and capped.

    The sample solutions were then neutralized with concentrated hydrochloric acid, added dropwise until the pH decreased from 12.0-13.0 to 8.0-8.5. The pH was then reduced to 7.2-7.5 by adding dilute hydrochloric acid with a titrator. The sample solution was transferred to a 50-mL plastic volumetric flask and stored until analysis. Aliquots of 10 mL were removed from the volumetric flask and 1 mL of TISAB III was added to each aliquot, bringing the pH to 5.2-5.4. Reagent blanks were used as negative controls and standard solutions were prepared with fluoride concentrations of 0.100, 1.00, and 10.0 mg F/L. The standard and sample solutions were analyzed with a fluoride ion-selective electrode (9609BNWP and 960900 fluoride combination electrode, Orion Dual Star, pH/ISE Benchtop; Thermo Scientific, Waltham, MA, USA). The electrode was rinsed and blotted dry before each measurement.

      >  Measuring fluoride levels by organic acid extraction

    Aliquots of 0.5-2.0 g of lyophilized FK were weighed into plastic beakers. To each aliquot was added 50-200 mL of 0.01-0.1 M citric acid and the mixtures were stirred with an agitator for 5-30 min. The mixtures were then centrifuged, filtered, and adjusted to pH 7.2-7.5 with a NaOH solution. Five milliliters of each filtrate was placed into a 10-mL volumetric flask. One milliliter of a TISAB III solution was added and the fluoride concentration was measured using the fluoride ion-selective electrode described above.

      >  Percent recovery using fluoride standard solutions

    To a beaker were added 0.5 g samples of FK and KM, an aliquot of fluoride standard solution, and 50 mL of 0.01 M citric acid. The mixture was then stirred for 5 min and filtered. Tenmilliliter aliquots of filtrate were removed from the beaker, adjusted to pH to 7.2-7.5 with 0.1 M NaOH, and transferred to a 10-mL volumetric flask. One milliliter TISAB III was added the flask and the fluoride concentration was measured with an ion-selective electrode as above. Percent recovery test was calculated as follows:

    Percent recovery of added fluoride = (F2 - F1)/F3 × 100%

    F1: Fluoride content of the sample

    F2: Fluoride content of the sample after adding the fluoride standard

    F3: Amount of added fluoride

      >  Statistical analyses

    All of the measurements performed on powders of KB, KM, and BK were done in triplicate. The results of each measurement are expressed as the mean ± SD. Analysis of variance (ANOVA) and Duncan’s multiple range tests were used to determine statistical significance at P < 0.05.

    Results and Discussion

      >  Type and concentration of organic acids

    Fig. 1 shows the changes in fluoride concentration of solutions extracted from lyophilized FK samples as a function of acid concentration. Citric, acetic, tartaric, lactic, oxalic, succinic, malic, and fumaric acids were evaluated for their ability to remove fluoride from lyophilized FK.

    The degree of fluoride removal increased significantly with increasing organic acid concentration from 0.001 to 0.1 M. Citric acid was particularly effective at extracting fluorine from lyophilized FK, although malic, tartaric, and lactic acids were also effective at a concentration of 1.0 M. Above 0.1 M citric acid, however, the amount of extracted fluoride remained the same. Most of the organic acids exhibited their highest extraction efficiency at concentrations between 0.1 and 0.3 M. Succinic, lactic, oxalic, and fumaric acids exhibited steadily increasing efficiencies from 0.1 to 1.0 M, but yielded unstable extraction efficiencies at higher concentrations. Among the eight organic acids, citric acid was the most effective in extracting fluoride from lyophilized FK.

      >  Extraction time and temperature

    The amount of fluoride extracted from FK increased with increasing extraction time until reaching a plateau at 300 s (Fig. 2).

    Therefore, a 5-min extraction time was deemed sufficient with 0.1 M citric acid solution. Kumagai (1984) reported that the extraction of fluoride from krill using 0.05 N HClO4 at room temperature was constant over 30 min. The effects of temperature on fluoride extraction with citric acid were also evaluated from 20℃ to 90℃. No significant difference (P > 0.05) was observed in extraction efficiency as a function of temperature (Table 1).

      >  Concentration of citric acid in the extraction solution

    Lyophilized FK was treated with 0.001-0.10 M citric acid and the fluoride concentrations of the resulting extracts were measured (Fig. 3). With 1.0 g of FK and 50 mL of citric acid solution, fluoride levels in the extracted solutions increased with increasing citric acid concentration from 0.001 M until reaching a plateau at 0.01 M. Thus, the optimum concentration of citric acid for fluoride extraction was 0.01 M.

      >  Volume of the extraction solution

    Fig. 4 shows the fluoride removed from lyophilized FK as a function of citric acid extraction solution volume from 15 to 150 mL. The extraction was performed for 5 min on a 1g sample. Extracted fluoride levels increased with the volume of the extraction solution from 15 to 50 mL. No further increase

    was observed at volumes greater than 50 mL. Thus, the optimum volume of 0.01 M citric acid solution for the extraction of fluoride from lyophilized FK was 50 mL.

      >  Amount of sample

    To optimize sample size for fluoride extraction, fluoride concentrations were measured after 5-min extractions (with 50 mL of 0.01 M citric acid) of 0.1-3.0 g lyophilized FK (Fig. 5). The amount of fluoride eluted increased from 0.1 to 0.5 g and then decreased with increasing sample sizes above 0.5 g (Fig. 5). Therefore, with extractions performed using the aforementioned optimized parameters 0.3-0.5 g of sample were ideal.

      >  Percent recovery of fluoride from krill by standard additions

    The percent recovery of fluoride (as determined by standard additions) from lyophilized FK and KM are given in Table 2. Recoveries of spiked fluoride (150 and 300 μg fluoride added to each sample) were 95.6 ± 4.7% and 99.5 ± 5.7% for lyophilized FK and 97.5 ± 1.8% and 101.3 ± 2.9% for lyophilized KM, respectively. Thus, recoveries were acceptable with a range of 95.6-101.3%. Kumagai (1984) reported recoveries of fluoride ranging from 93.3% to 96.9% for 18 samples of fluoride extract using an ion-selective electrode. Their extraction procedure consisted of shaking 1-2 g of sample in 100 mL of 0.05 N HClO4 for 45-60 min. Malde et al. (2001) reported fluoride recoveries from 80% and 110%. These data agree with the values obtained in the current study, although the present study used a lower ionic strength of citric acid and a shorter extraction time. No significant differences were observed in percent recovery between the samples and spiked fluoride solutions used in the present study.

      >  Comparison of fluoride content as determined by alkali fusion methods and citric acid extraction

    The fluoride contents of lyophilized FK, KM, and BK determined by alkali fusion methods and by citric acid extraction are given in Table 3. Levels of 691.46 ± 38.48, 296.58 ± 23.30, and 546.61 ± 16.23 ppm (dry matter), respectively, were obtained using alkali fusion methodsf 705.23 ± 20.38, 297.78 ± 12.77, and 575.11 ± 41.76 ppm (dry matter) were obtained using citric acid extraction for FK, KM, and BK, respectively. The two methods yielded statistically identical results (P > 0.05), thereby indicating that the fluoride in krill can be extracted using citric acid.

    Fluorine levels in frozen krill and krill meal were 1041.9 ± 27.7 and 1942.6 ± 48.1 ppm (dry matter), respectively. These high fluorine levels have been associated with the ability of krill to live in below-freezing waters (Soevik and Braekkan, 1979). For E. superba, whole-body fluorine levels were 1,137 mg F/kg dry weight (Malde et al., 2001) and 490 μg/g wet weight (Kumagai, 1984) have been reported. Additional studies have reported levels of 215 ppm in raw muscle (Park et al., 1988), 1,920.4 ppm in whole body, 99 mg F/kg dry weight in muscle (Kim et al., 1990), 2,400 ppm in whole body, and 570 mg F/kg dry matter in muscle (Soevik and Braekkan, 1979).

    The amount of soluble protein in krill muscle depends on the pH. The amount of extracted fluorine depends on the ionic strength of, and the types of ionic species in, the extracting solution. The amount of fluoride extracted from krill decreased from 64.5 to 8 ppm when extracting with an ionic strength of 0.05 M NaCl at pH 11 (Park et al., 1988). An electrocondensation method using an aluminum electrode was also developed as a means of removing excess fluoride from Antarctic krill. For the chitinous components of Antarctic krill, 30-70% of the total fluoride could be removed after 120 min of electrocondensation (Kim et al., 1990).

    The results of the current study show that citric acid extraction was just as effective as the methods of Park et al. (1988) and Kim et al. (1990) for reducing the amount of fluoride in prepared krill samples. Citric acid is a common food additive that is considered safe for consumption throughout the food industry. In this study, the most effective extraction of fluoride from krill was achieved using 50 mL of 0.01 M citric acid for 5 min on 0.3-0.5 g samples.

    Although present legislation in the Republic of Korea has set the maximum fluorine level for drinking water at 2.0 mg/L, 100 μg/g for fish protein concentrates is permitted by the US Food and Drug Administration (US FDA), and up to 2.5 and 4.0 mg of fluorine per day are considered safe dietary intakes for children and adults, respectively (National Academy of Sciences, 1989; Brown, 1990). A current need exists to determine acceptable limits of fluoride in various foods and to define maximum allowable levels of dietary fluoride prior to using krill biomass as a nutritional supplement for humans.

  • 1. Arthur BD 1971 Biologic Effects of Atmospheric Pollutants, Fluoride. google
  • 2. Brown ML 1990 Present Knowledge in Nutrition. P.274-278 google
  • 3. Filho TA 1993 Fluorine removal during production of krill paste and krill protein concentrates. [Acta Aliment] Vol.22 P.269-281 google
  • 4. Kim KH, Kim DM, Kim YH, Yoon HH 1990 Fluoride migration of frozen antarctic krill according to thawing methods. [Korean J Food Sci Technol] Vol.22 P.168-171 google
  • 5. Kumagai M 1984 Simple and rapid method for determination of fluoride in Antarctic krill. [Bull Jpn Soc Sci Fish] Vol.50 P.1877-1881 google
  • 6. Malde MK, Bjorvatn K, Julshamn K 2001 Determination of fluoride in food by the use of alkali fusion and fluoride ion-selective electrode. [Food Chem] Vol.73 P.373-379 google
  • 7. 1989 Recommended Dietary Allowances. P.284 google
  • 8. Nicol S, Foster J. 2003 Recent trends in the fishery for Antarctic krill. [Aquat Living Resour] Vol.16 P.42-45 google
  • 9. Nicol S, Forster I, Spence J 2000 Products derived from krill. In: Krill: Biology, Ecology and Fisheries. Everson I, ed. P.265-268 google
  • 10. Park HJ, Ham KS, Kim DM, Kim KH 1988 Decrease of fluoride content of antarctic krill. [Korean J Food Sci Technol] Vol.20 P.19-22 google
  • 11. Soevik T, Braekkan OR 1979 Fluoride in Antarctic krill (Euphausia superba) and Atlantic krill (Meganyctiphanes norvegica). [J Fish Res Board Can] Vol.36 P.1414-1416 google
  • 12. Szewielow A 1981 Fluoride in krill (Euphausia superba Dana). [Meeresforschung] Vol.28 P.244-246 google
  • 13. Zhang H, Pan J, Cheng X, Zhu B 1993 Biogeochemistry research of fluoride in Antarctic ocean. I. The study of fluoride anomaly in Antarctic krill. [Antarct Res] Vol.4 P.55-61 google
  • [Fig. 1.] Effect of various kinds and concentration of organic acids on extraction of fluoride in krill.
    Effect of various kinds and concentration of organic acids on extraction of fluoride in krill.
  • [Fig. 2.] Effect of time on the extraction of fluoride in krill.
    Effect of time on the extraction of fluoride in krill.
  • [Fig. 3.] Effect of citric acid volume on the extraction of fluoride in krill.
    Effect of citric acid volume on the extraction of fluoride in krill.
  • [Table 1.] Effect of temperature of citric acid solution on fluoride contents in krill Euphausia superba
    Effect of temperature of citric acid solution on fluoride contents in krill Euphausia superba
  • [Fig. 4.] Effect of citric acid volume on the extraction of fluoride in krill.
    Effect of citric acid volume on the extraction of fluoride in krill.
  • [Table 2.] Recoveries of fluoride from frozen krill and krill meat (dry basis)
    Recoveries of fluoride from frozen krill and krill meat (dry basis)
  • [Table 3.] Comparisons of fluoride contents obtained by alkali fusion method and by citric acid extraction (dry basis)
    Comparisons of fluoride contents obtained by alkali fusion method and by citric acid extraction (dry basis)
  • [Fig. 5.] Effect of sample weight on the extraction of fluoride in krill.
    Effect of sample weight on the extraction of fluoride in krill.