Redescription of Two Urostylid Ciliates (Ciliophora: Urostylida), Anteholosticha pulchra and Metaurostylopsis struederkypkeae from Korea

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  • ABSTRACT

    Two urostylid ciliates, Anteholosticha pulchra (Kahl, 1932) Berger, 2003 and Metaurostylopsis struederkyp-keae Shao et al., 2008, new to Korea, were collected from the Yellow Sea and the East Sea, Korea, respec-tively. They were identified based on live observation and protargol impregnation. Taxonomical characters of A. pulchra are as follows: 190-300×30-55 μm size in vivo; contractile vacuole located on the left side of the posterior 1/4 of the cell; spherical-reddish granules at cirral bases and around dorsal bristles, somewhat sparsely distributed throughout the cell surface; four frontal and two frontoterminal cirri; four dorsal kineties;caudal cirri absent. Metaurostylopsis struederkypkeae is characterized as follows: 80-110×40-50 μm size in vivo; caudal cirri absent; two types of cortical granules: type 1, yellow-green arranged along the ventral cirral rows and dorsal kineties; type 2, small and reddish, with an irregular arrangement; four frontal, four to eight frontoterminal, and two to six transverse cirri; five to seven left and three to five right marginal rows. Sequences of small subunit ribosomal DNA were determined from both species, and pairwise distances with their relatives were analyzed.


  • KEYWORD

    Anteholosticha pulchra , Metaurostylopsis struederkypkeae , urostylid ciliate , marine , Korea

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  • [Table 1.] Comparison of morphometric data from Anteholosticha pulchra with Li et al. (2007)
    Comparison of morphometric data from Anteholosticha pulchra with Li et al. (2007)
  • [Fig. 1.] Morphology of Anteholosticha pulchra and Metaurostylopsis struederkypkeae from live (A-C F-J) and protargol impregnated (D E K L) specimens. A-E Anteholosticha pulchra: A Ventral view of live contractile vacuole (arrow); B C Pattern of cortical granules in dorsal and ventral views reddish cortical granules (arrow); D E Cirral base in ventral and dorsal views from protargol impregnation; F-L Metaurostylopsis struederkypkeae: F Ventral view of live contractile vacuole (arrow); G H Ventral and dorsal views yellow-green cortical granules (arrow) small reddish cortical granules (arrowhead); I J Ventral and dorsal views which show the arrangement of yellow-green cortical granules; K L Cirral base on ventral and dorsal views with protargol impregnation. AZM adoral zone of membranelles; BC buccal cirrus; DK dorsal kineties; EM endoral membrane; FC frontal cirri; FTC frontoterminal cirri; LMR left marginal row; Ma macronuclear nodules; MP midventral pairs; MVR midventral row; PM paroral membrane; RMRright marginal row; TC transverse cirri. Scale bars: A-E=100 μm F-L=50 μm.
    Morphology of Anteholosticha pulchra and Metaurostylopsis struederkypkeae from live (A-C F-J) and protargol impregnated (D E K L) specimens. A-E Anteholosticha pulchra: A Ventral view of live contractile vacuole (arrow); B C Pattern of cortical granules in dorsal and ventral views reddish cortical granules (arrow); D E Cirral base in ventral and dorsal views from protargol impregnation; F-L Metaurostylopsis struederkypkeae: F Ventral view of live contractile vacuole (arrow); G H Ventral and dorsal views yellow-green cortical granules (arrow) small reddish cortical granules (arrowhead); I J Ventral and dorsal views which show the arrangement of yellow-green cortical granules; K L Cirral base on ventral and dorsal views with protargol impregnation. AZM adoral zone of membranelles; BC buccal cirrus; DK dorsal kineties; EM endoral membrane; FC frontal cirri; FTC frontoterminal cirri; LMR left marginal row; Ma macronuclear nodules; MP midventral pairs; MVR midventral row; PM paroral membrane; RMRright marginal row; TC transverse cirri. Scale bars: A-E=100 μm F-L=50 μm.
  • [Fig. 2.] Micrographs of Anteholosticha pulchra from live (A-E) and protargol impregnated (F-L) specimens. A B Live ventral (A) anddorsal (B) view arrangement of cortical granules (arrowhead); C Ventral view contractile vacuole (arrow); D Dorsal view reddishcortical granules (arrow); E Live ventral view of transverse cirri; F G Dorsal and ventral views of protargol-impregnated specimen; H Ventral view the frontal (arrows) and frontoterminal (arrowhead) cirri; I Buccal cirri (arrow); J Ventral view the transversecirri; K Dorsal view the dorsal kineties typically four; L Macronuclear nodules (arrow). Scale bars: A B F G=100 μm.
    Micrographs of Anteholosticha pulchra from live (A-E) and protargol impregnated (F-L) specimens. A B Live ventral (A) anddorsal (B) view arrangement of cortical granules (arrowhead); C Ventral view contractile vacuole (arrow); D Dorsal view reddishcortical granules (arrow); E Live ventral view of transverse cirri; F G Dorsal and ventral views of protargol-impregnated specimen; H Ventral view the frontal (arrows) and frontoterminal (arrowhead) cirri; I Buccal cirri (arrow); J Ventral view the transversecirri; K Dorsal view the dorsal kineties typically four; L Macronuclear nodules (arrow). Scale bars: A B F G=100 μm.
  • [Table 2.] Comparison of morphometric data from Metaurostylopsis struederkypkeae with Shao et al. (2008)
    Comparison of morphometric data from Metaurostylopsis struederkypkeae with Shao et al. (2008)
  • [Fig. 3.] Micrographs of Metaurostylopsis struederkypkeae from live (A-D) and protargol impregnated (E-H) specimen. A B Live ven-traland dorsal views; C Contractile vacuole (arrow); D Yellow-green cortical granules (arrow) small reddish cortical granules (arrow-head); E Ventral view of protargol-impregnated specimen; F Ventral view left and right marginal rows; G Dorsal view typicallythree dorsal kineties (arrows) and two dikinetids (arrowheads); H Frontoterminal (arrow) and buccal (arrowhead) cirri. Scale bars: A-C E=50 ㎛.
    Micrographs of Metaurostylopsis struederkypkeae from live (A-D) and protargol impregnated (E-H) specimen. A B Live ven-traland dorsal views; C Contractile vacuole (arrow); D Yellow-green cortical granules (arrow) small reddish cortical granules (arrow-head); E Ventral view of protargol-impregnated specimen; F Ventral view left and right marginal rows; G Dorsal view typicallythree dorsal kineties (arrows) and two dikinetids (arrowheads); H Frontoterminal (arrow) and buccal (arrowhead) cirri. Scale bars: A-C E=50 ㎛.
  • [Table 3.] Kimura two-parameter pairwise distances (%) among the five species in Anteholosticha-Nothoholosticha assemblage basedon small subunit ribosomal DNA sequences
    Kimura two-parameter pairwise distances (%) among the five species in Anteholosticha-Nothoholosticha assemblage basedon small subunit ribosomal DNA sequences
  • [Table 4.] Kimura two-parameter pairwise distance (%) based on small subunit ribosomal DNA sequences
    Kimura two-parameter pairwise distance (%) based on small subunit ribosomal DNA sequences