Effect of Lithospermi Radix on Contact Dermatitis Induced by Dinitrofluorobenzene in Mice

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  • ABSTRACT

    Objectives

    The root of Lithospermum erythrorhizon Sieb. et Zucc. (Lithospermi Radix, LR) is a kind of heat clearing and blood cooling medicinal herbs. It can clear away heat and cool the blood, reduce toxins and disperse maculae. LR has long been used as efficacious therapy for inflammation, burns, frostbite and skin diseases such as eczema and psoriasis.

    Methods

    In the present study, we investigate anti-allergic and anti-inflammatory effects of LR by using the 1-fluoro-2, 4- dinitrofluorobenzene (DNFB)-induced contact dermatitis mouse model.

    Results

    Topical application of 10 mg/mL of LR effectively inhibited skin lesions induced by repeated paintings with DNFB. Topical application of LR also inhibited hyperplasia, edema, spongiosis and infiltrations of mononuclear cells. In addition, production levels of total immunoglobulin and IgG1 in serum were decreased by using LR in vivo.

    Conclusions

    These data suggest that LR acts as an antiinflammatory agent, improving skin lesions in CD mice.


  • KEYWORD

    Lithospermi Radix , dinitrofluorobenzene , contact dermatitis , anti-allergic

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  • [Figure 1] Experimental design. The experimental groups except the naive group were sensitized by painting with DNFB on day 1, 2 and 3. Then, they were challenged on day 7, 9, 11, 13 by painting with DNFB. The naive group was treated with a vehicle (acetone and olive oil, AOO) in the same way. Lithospermi Radix (LR) was topically painted from day 8 to 13. All animals were sacrificed on day 14.
    Experimental design. The experimental groups except the naive group were sensitized by painting with DNFB on day 1, 2 and 3. Then, they were challenged on day 7, 9, 11, 13 by painting with DNFB. The naive group was treated with a vehicle (acetone and olive oil, AOO) in the same way. Lithospermi Radix (LR) was topically painted from day 8 to 13. All animals were sacrificed on day 14.
  • [Figure 2] Effects of LR on skin lesions in CD mice. Skin conditions were observed using a skin image analysis system. (A) Naive mice, treated with a vehicle and painted with a vehicle; (B) CD-induced control (CTL) mice, were sensitized and challenged with DNFB and then painted with a vehicle; (C) Lithospermi Radix (LR)-treated CD mice, sensitized and challenged with DNFB and then painted with 10 mg/mL of LR solution for 6 days (x30).
    Effects of LR on skin lesions in CD mice. Skin conditions were observed using a skin image analysis system. (A) Naive mice, treated with a vehicle and painted with a vehicle; (B) CD-induced control (CTL) mice, were sensitized and challenged with DNFB and then painted with a vehicle; (C) Lithospermi Radix (LR)-treated CD mice, sensitized and challenged with DNFB and then painted with 10 mg/mL of LR solution for 6 days (x30).
  • [Figure 3] Effects of LR on histopathological changes in CD mice. Tissues were stained with hematoxylin and eosin and were observed using a light microscope. (A) Naive mice, treated with a vehicle and painted with a vehicle; (B) CD-induced control (CTL) mice, sensitized and challenged with DNFB and then painted with a vehicle; (C) Lithospermi Radix (LR)- treated CD mice, sensitized and challenged with DNFB and then painted with 10 mg/mL of LR solution for 6 days (x100).
    Effects of LR on histopathological changes in CD mice. Tissues were stained with hematoxylin and eosin and were observed using a light microscope. (A) Naive mice, treated with a vehicle and painted with a vehicle; (B) CD-induced control (CTL) mice, sensitized and challenged with DNFB and then painted with a vehicle; (C) Lithospermi Radix (LR)- treated CD mice, sensitized and challenged with DNFB and then painted with 10 mg/mL of LR solution for 6 days (x100).
  • [Figure 4] Effects of LR on production levels of antibody in CD mice. Production levels of antibodies in serum were measured using the ELISA method. Naive: non-treated normal mice, Control: non-treated CD mice, and LR: CD mice treated with 10 mg/mL of LR. (A) total antibody, (B) IgG1, and (C) IgG2a. All values are presented as mean ± SD. ##P?0.01, ###P?0.01 vs. naive mice, and *P?0.05 vs. non-treated CD mice (Control) (n = 8).
    Effects of LR on production levels of antibody in CD mice. Production levels of antibodies in serum were measured using the ELISA method. Naive: non-treated normal mice, Control: non-treated CD mice, and LR: CD mice treated with 10 mg/mL of LR. (A) total antibody, (B) IgG1, and (C) IgG2a. All values are presented as mean ± SD. ##P?0.01, ###P?0.01 vs. naive mice, and *P?0.05 vs. non-treated CD mice (Control) (n = 8).